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目的:建立反相高效液相色谱法监测复方磺胺甲噁唑在呼吸道感染病人中的血药浓度。方法:C_(18)填料柱(220mm×4.6mm),流动相为水:甲醇(66∶44),流速1.0ml·min~(-1),检测波长223nm。结果:磺胺甲噁唑(SMZ)与甲氧苄啶(TMP),线性范围各为2～25μg·ml~(-1)、0.5～10μg·ml~(-1),平均回收率100.3％,日内变异<5％。8名上呼吸道感染病人口服SMZ_(Co)1.0g,bid,治疗期间监测SMZ、TMP稳态峰浓度分别为(99.1±45.4)和(2.4±0.9)μg·ml~(-1),稳态谷浓度分别为(63.4±39.2)和(1.4±0.7)μg·ml~(-1)。结论:应用复方磺胺甲噁唑期间应监测血药浓度,可用RP-HPLC法作血药浓度监测。
Objective: To establish a reversed-phase high performance liquid chromatography (HPLC) method for the monitoring of plasma concentration of sulfamethoxazole in patients with respiratory tract infection. Methods: C18 column (220mm × 4.6mm) was used. The mobile phase consisted of water and methanol (66:44). The flow rate was 1.0ml · min ~ (-1) and the detection wavelength was 223nm. Results: The linear range of SMZ and TMP was 2 ~ 25μg · ml -1, 0.5 ~ 10μg · ml -1, the average recovery was 100.3% Intraday variation <5%. Eight patients with upper respiratory tract infection were given oral SMZ_ (Co) 1.0g for bid. The steady-state peak concentrations of SMZ and TMP during monitoring were (99.1 ± 45.4) and (2.4 ± 0.9) μg · ml -1, respectively The trough concentrations were (63.4 ± 39.2) and (1.4 ± 0.7) μg · ml -1, respectively. Conclusion: The plasma concentration of sulfamethoxazole should be monitored during the course of the compound sulfamethoxazole. RP-HPLC can be used to monitor the blood concentration.