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目的探讨中药方剂何首乌饮对衰老小鼠卵巢PCNA和端粒酶活性的影响。方法 D-半乳糖连续腹腔注射致亚急性衰老动物模型,造模后灌胃何首乌饮连续32 d,小鼠断头处死取卵巢。采用免疫组织化学分析方法和PCRELISA法检测小鼠卵巢组织PCNA、端粒酶活性情况。结果模型组小鼠卵巢PCNA阳性细胞数低于正常对照组小鼠(P<0.05)。何首乌饮延缓衰老各剂量组PCNA阳性细胞数高于模型组(P<0.05)。模型组小鼠卵巢组织端粒酶活性明显低于正常对照组小鼠(P<0.05)。何首乌饮延缓衰老组端粒酶活性均低于模型组(P<0.05),并呈现一定的剂量依赖性,差异有统计学意义(P<0.05)。结论何首乌饮可明显提高衰老小鼠卵巢的增殖能力和端粒酶活性,具有一定的延缓衰老作用。
Objective To investigate the effect of Heshouwuyin on ovarian PCNA and telomerase activity in aging mice. Methods D-galactose was injected intraperitoneally into the animal model of subacute aging. After the model was given, the Polygonum multiflorum Thunb was continuously instilled for 32 days. The mice were decapitated and ovariectomized. Immunohistochemistry and PCR-ELISA were used to detect PCNA and telomerase activity in mouse ovarian tissue. Results The ovarian PCNA positive cells in model group were lower than those in normal control group (P <0.05). The content of PCNA positive cells in each dose group of Polygonum hydropiper beverage was higher than that in model group (P <0.05). The telomerase activity in the model group was significantly lower than that in the normal control group (P <0.05). The telomerase activity of the HLJ group was lower than that of the model group (P <0.05), and showed a dose-dependent manner, the difference was statistically significant (P <0.05). Conclusion Polygonum multiflorum drink can significantly increase the ovarian proliferation and telomerase activity in aging mice, and has a certain anti-aging effect.