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目的对41例人胃癌和17例癌旁组织中的金属蛋白酶MMP－2，MMP－9及金属蛋白酶抑制剂TIMP－1基因表达进行了检测；方法应用免疫组织化学（SP）法和原位杂交（cDNA－mRNA）技术对41例胃癌及癌旁组织的金属蛋白酶及其抑制剂进行检测；结果MMP－2及MMP－9蛋白在胃癌及癌旁组织阳性率分别是73．1％（30／41），65．8％（27／41）和22．5％（4／17），41．1％（7／17）。胃癌及癌旁组织的TIMP－1的表达分别是56．9％（23／41）及34．6％（6／17），且MMP－9／TIMP－1的比值与胃癌的侵袭转移呈正相关。一致性检验显示基因的免疫组化和原位杂交检测结果的关系密切（P＜0．05）；结论MMPS基因的过度表达参与了胃癌的发生发展过程，同时证明TIMP基因的表达参与了肿瘤细胞的生物学行为的改变过程，MMPs／TIMP基因的平衡表达及免疫组化和原位杂交是检测胃癌组织侵袭，转移的可靠方法，本实验的结果为建立肿瘤转移的分子标志提供了理论和实验依据。
Objective To detect the expression of metalloproteinase MMP-2, MMP-9 and metalloproteinase inhibitor TIMP-1 in 41 cases of human gastric cancer and 17 cases of adjacent tissues. Methods Immunohistochemistry (SP) and in situ hybridization were used. (cDNA-mRNA) technology detected metalloproteinases and their inhibitors in 41 cases of gastric cancer and paracancerous tissues; the positive rate of MMP-2 and MMP-9 protein in gastric cancer and adjacent tissues was 73.1% (30/ 41), 65.8% (27/41) and 22.5% (4/17), 41.1% (7/17). The expression of TIMP-1 in gastric cancer and adjacent tissues was 56.9% (23/41) and 34.6% (6/17) respectively, and the ratio of MMP-9/TIMP-1 was positively correlated with the invasion and metastasis of gastric cancer. . The consistency test showed that the immunohistochemistry of the gene was closely related to the results of in situ hybridization (P<0.05). Conclusion The overexpression of MMPS gene is involved in the development of gastric cancer, and the expression of TIMP gene is involved in the tumor cells. The process of biological behavioral changes, balanced expression of MMPs/TIMP genes, and immunohistochemistry and in situ hybridization are reliable methods for detecting invasion and metastasis of gastric cancer tissues. The results of this experiment provide theoretical and experimental evidence for the establishment of molecular markers of tumor metastasis. in accordance with.