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目的:建立并验证镇静小鼠气道反应性的非侵入式测定法,探讨小鼠气道高反应性与气道炎症的关系。方法:观察致敏及药物对小鼠引喘阈浓度的影响,及支气管肺泡灌洗液(BALF) 中白细胞渗出量的变化。结果:与未致敏小鼠相比,致敏小鼠吸入OA6h 的MCh 引喘阈浓度显著降低,BALF 中白细胞渗出量显著增高,地塞米松(7 .5mgkg) 和氨茶碱(37 .5 mgkg) 可降低致敏小鼠吸入OA 引起的气道反应性增高和BALF 中白细胞渗出的增加。结论:本模型可有效地检测小鼠气道反应性,抗原引起的小鼠气道高反应性与气道炎症有关。
Objective: To establish and validate the non-invasive assay of airway responsiveness in sedated mice and to explore the relationship between airway hyperresponsiveness and airway inflammation in mice. Methods: The effects of sensitization and drugs on threshold of asthma in mice and the leukocyte exudation in bronchoalveolar lavage fluid (BALF) were observed. Results: Compared with non-sensitized mice, sensitized mice inhaled OA6h MCh asthma threshold decreased significantly, leukocyte exudation was significantly increased in BALF, dexamethasone (7.5mgkg) and aminophylline 37.5 mg kg-1) reduced the increased airway responsiveness induced by inhaled OA in sensitized mice and increased leukocyte exocytosis in BALF. Conclusion: This model can effectively detect the airway responsiveness in mice. Antigen-induced airway hyperresponsiveness in mice is associated with airway inflammation.