论文部分内容阅读
雌激素替代疗法对更年期骨质疏松的预防及治疗效果显著，但作用机理不很明确，本研究通过体外培养的人类成骨细胞ＨＯＳＴＥ８５，观察雌二醇对成骨细胞增殖及Ⅰ型胶原产生的影响。方法：在体外培养的人类成骨细胞ＨＯＳＴＥ８５培养液中，加入雌二醇（０．０１－１０ｎｍｏｌ／Ｌ），分别培养２４、４８、７２及９６ｈ，于结束培养前６ｈ，加入３Ｈ－胸腺脱氧核苷检测细胞增殖；或于细胞开始培养时，加入３Ｈ－脯氨酸检测Ｉ型胶原产生。结果：在２４ｈ时，雌二醇刺激细胞增殖，但与对照组无显著差异；在４８、７２及９６ｈ时，雌二醇抑制细胞增殖。但无明显量效关系。在４８、７２ｈ时，雌二醇明显刺激细胞Ｉ型胶原产生，量效关系显著，在９６ｈ时。也能刺激Ｉ型胜原产生。结论：雌二醇对人类成骨细胞ＨＯＳＴＥ８５增殖具有短期刺激，长期抑制的作用，对Ｉ型胶原产生具有促进作用，这可能是其临床治疗作用的主要机制。
Estrogen replacement therapy for menopausal osteoporosis prevention and treatment significantly, but the mechanism is not clear, the present in vitro study of human osteoblasts HOSTE85, estradiol was observed on osteoblast proliferation and collagen production type Ⅰ influences. Methods: Human osteoblasts cultured broth HOSTE85 added estradiol (0.01-10nmol / L), were cultured 24,48,72 and 96h, prior to completion of culture 6h, was added deoxygenated 3H- thymus Nucleoside detection of cell proliferation; or in the beginning of cell culture, adding 3H-proline to detect type I collagen production. Results: Estradiol stimulated cell proliferation at 24h, but no significant difference with the control group; at 48, 72 and 96h, estradiol inhibited cell proliferation. However, there is no obvious relationship between the quantity and the effect. At 48 and 72h, estradiol obviously stimulated the production of type I collagen, and the dose-response relationship was significant at 96h. I can also stimulate type Sheng wins. Conclusion: estradiol on human osteoblast proliferation HOSTE85 short-term stimulus and long-term suppression effect, produce type I collagen can promote, this may be the main mechanism of its clinical therapeutic effect.