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目的分析探讨高危型人乳头瘤病毒(HPV)多重核酸扩增荧光检测技术在宫颈癌筛查中的应用效果。方法将2013年2月至2014年4月期间本院中28~58岁的合格妇女210例纳入研究对象,对其进行宫颈癌筛查。结果本次研究中的210例妇女,宫颈癌及宫颈鳞状上皮内高度病变占总数的9.53%;多重核酸扩增荧光检测和第二代杂交捕获试验的准确性和有效性趋向一致;多重核酸扩增荧光检测和第二代杂交捕获试验的HPV阳性率具有较好的一致性。结论高危型HPV多重核酸扩增荧光检测技术具有较高灵敏度和特异度,与第二代杂交捕获试验相比具有良好的一致性,值得在临床中推广使用。
Objective To investigate the application effect of high-risk human papillomavirus (HPV) multiplex nucleic acid amplification fluorescence detection in cervical cancer screening. Methods A total of 210 eligible women aged 28-58 years from February 2013 to April 2014 in our hospital were enrolled in the study and screened for cervical cancer. Results 210 cases of women in this study, cervical cancer and cervical squamous intraepithelial lesion accounted for 9.53% of the total number; multiple nucleic acid amplification fluorescence detection and second-generation hybridization capture test accuracy and validity tend to be the same; multiple nucleic acid The positive rate of HPV by amplification fluorescence detection and the second generation hybridization capture test has good consistency. Conclusion High-risk HPV multiplex nucleic acid amplification fluorescence detection technology with high sensitivity and specificity, compared with the second generation hybridization capture test has good consistency, it is worth in the clinic to promote the use.