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目的探讨病原体灭活技术(PRT)是否对血小板源性细胞因子的释放产生影响。方法随机选取捐献血小板的献血者20人,取单采血小板标本60 m L/人(份),全部平均分成2份(组),实验组:20份采用维生素B2(终浓度为50μmol/L)联合紫外光照射(波长265-370 nm,剂量6.2 J/m L)光化学法照射8.5 min;对照组:20份不做处理。2组标本均放置于(22±2)℃水平振荡条件下保存7 d,分别于1、3、5和7 d取样6 m L,检测其血小板计数(Plt)、血小板分布宽度(PDW)、平均血小板体积(MPV)以及血小板源性细胞因子(CCL3、CCL5、TGF-β-1和PF4)含量。结果在保存的7 d过程中,与对照组相比,实验组中Plt略微降低,MPV和PDW略微升高(P>0.05)。实验组与对照组比较,在7 d时,CCL3含量(pg/m L)为11.90±0.4 vs 10.97±0.51(P<0.05),CCL5含量(ng/m L)为190.10±15.03 vs 150.02±20.0(P<0.05);在5和7 d时,TGF-β-1含量(ng/m L)分别为22.18±2.36 vs 21.15±1.43、37.21±3.01 vs 34.11±2.03(P<0.05),PF4含量(mg/L)分别为7.87±1.05 vs 5.75±0.91、9.01±1.11 vs 6.13±1.07(P<0.05)。结论随着血小板贮存时间的延长,血小板源性细胞因子的积累逐渐增加;在血小板保存末期,VB2-PRT处理明显增加血小板源性细胞因子的积累。
Objective To investigate whether pathogen inactivation (PRT) affects the release of platelet-derived cytokines. Methods Totally 20 blood donors were donated for platelet donation, 60 mL / human apheresis platelets were randomly divided into 2 groups (experimental group). The experimental group was given vitamin B2 (final concentration was 50 μmol / L) Combined with ultraviolet light (wavelength 265-370 nm, dose 6.2 J / m L) photochemical irradiation 8.5 min; control group: 20 not treated. The two groups of specimens were stored at (22 ± 2) ℃ for 7 days and then sampled for 6 days at 1,3,5 and 7 days respectively. The platelet count (Plt), platelet distribution width (PDW) Mean platelet volume (MPV) and platelet-derived cytokines (CCL3, CCL5, TGF-β-1 and PF4). Results Compared with the control group, Plt in the experimental group decreased slightly and the MPV and PDW slightly increased (P> 0.05) during the 7 days of storage. Compared with the control group, CCL3 content (pg / m L) was 11.90 ± 0.4 vs 10.97 ± 0.51 (P <0.05) and CCL5 content (ng / m L) was 190.10 ± 15.03 vs 150.02 ± 20.0 (P <0.05). At 5 and 7 d, the levels of TGF-β-1 (ng / m L) were 22.18 ± 2.36 vs 21.15 ± 1.43, 37.21 ± 3.01 vs 34.11 ± 2.03 (mg / L) were 7.87 ± 1.05 vs 5.75 ± 0.91, 9.01 ± 1.11 vs 6.13 ± 1.07, respectively (P <0.05). Conclusion With the prolongation of platelet storage time, the accumulation of platelet-derived cytokines gradually increased. At the end of platelet preservation, VB2-PRT treatment significantly increased the accumulation of platelet-derived cytokines.