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目的研究茉莉酸甲酯(Me JA)和高温胁迫对白桦悬浮细胞三萜合成的影响。方法利用不同浓度(25、50、100、150μmol/L)Me JA和高温(50℃处理2 h)处理白桦细胞,进行生长量、细胞活力、抗逆酶活性、丙二醛和总三萜量及其合成关键酶基因相对表达量分析。结果高温和Me JA的复合处理对白桦细胞三萜合成的诱导作用比单独的Me JA和高温处理更强。高温胁迫1 d后加入150μmol/L的Me JA处理,细胞总三萜量最高,为76.6 mg/g,分别比空白对照、单独的Me JA和高温处理增加81.3%、159.9%和13.1%;三萜合成关键酶鲨烯合酶(SS)、鲨烯环氧酶(SE)、羽扇醇合酶(BPW)和β-香树酯醇合酶(BPY)基因的相对表达量也分别比空白对照增加297.1%、83.7%、1 032.6%和282.4%。高温胁迫1 d后加入Me JA处理,超氧化物歧化酶(SOD)和苯丙氨酸裂解酶(PAL)活性高于空白对照;生长量和细胞活力被抑制。结论在高温胁迫后加入Me JA的复合处理可能通过影响白桦细胞生长量、细胞活力、丙二醛量和防御酶活性变化,调节三萜合成关键酶基因的表达,最终促进三萜物质的高效合成与积累。
Aim To study the effects of methyl jasmonate (Me JA) and heat stress on the synthesis of triterpene in suspension cells of Betula platyphylla. Methods Betula platyphylla cells were treated with Me JA at different concentrations (25, 50, 100 and 150μmol / L) and high temperature (50 ℃ for 2 h), and the growth, cell viability, antitumor activity, malondialdehyde and total triterpene And the relative expression of its key enzyme genes. Results The induction of triterpene synthesis by Betula platyphylla cells with high temperature and Me JA treatment was stronger than Me JA alone and hyperthermia treatment. The highest total triterpene content was 76.6 mg / g, which was 81.3%, 159.9% and 13.1% higher than that of blank control, me JA alone and high temperature treatment, respectively, after being treated with 150 μmol / L Me JA for 1 d under high temperature stress. The relative expressions of squalene synthase (SS), squalene epoxidase (SE), lupoid alcohol synthase (BPW) and β-amyrin synthase (BPY) genes, An increase of 297.1%, 83.7%, 1 032.6% and 282.4% respectively. After treatment with Me JA for 1 day, the activity of superoxide dismutase (SOD) and phenylalanine lyase (PAL) was higher than that of the blank control; the growth and cell viability were inhibited. Conclusion The combination treatment of Me JA after high temperature stress may regulate the expression of key enzyme genes of tritolol by affecting the growth of cell, cell viability, the amount of malondialdehyde and the activity of defense enzymes, and finally promote the high-efficient synthesis of triterpenoids With accumulation.