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目的:研究泛素样蛋白UBQLN2在肺癌中的表达及对非小细胞肺癌细胞增殖、侵袭转移能力的影响。方法:采用免疫组化的方法检测非小细胞肺癌组织(24例)以及癌旁正常组织(24例)中UBQLN2的表达情况，分析UBQLN2表达水平与肺癌淋巴结转移之间的关系；运用实时荧光定量PCR和Western blot检测UBQLN2在人正常支气管肺上皮细胞和非小细胞肺癌细胞中的表达差异；利用慢病毒过表达技术结合体外MTS、EDU实验检测UBQLN2对非小细胞肺癌细胞增殖的影响；通过体外划痕实验、Transwell实验检测UBQLN2对非小细胞肺癌细胞侵袭转移的影响；通过GFP-LC3-RFP-mLC3质粒包装病毒构建双荧光自噬流检测体系和Western blot检测过表达UBQLN2后自噬的改变；最后利用TCGA在线数据库分析UBQLN2表达水平与肺癌患者预后的相关性。结果:UBQLN2的表达在癌旁肺组织中明显高于肺癌组织(n P<0.01)，且在淋巴结转移阴性患者中的表达显著高于淋巴结转移阳性患者(n P<0.01)；UBQLN2在非小细胞肺癌细胞相较正常肺上皮细胞中的表达明显减少，过表达UBQLN2可抑制肿瘤细胞的增殖及侵袭转移；且过表达UBQLN2可增强肿瘤细胞自噬能力；UBQLN2的表达与非小细胞肺癌患者预后呈正相关。n 结论:UBQLN2在非小细胞肺癌组织和细胞中明显低表达并与淋巴转移呈负相关；UBQLN2可抑制非小细胞肺癌细胞增殖及侵袭转移；UBQLN2的表达与患者预后呈正相关。“,”Objective:To study the expression of ubiquilin2 (UBQLN2) in non-small cell lung cancer (NSCLC) and its effect on the proliferation, invasion and metastasis ability of NSCLC cells.Methods:Immunohistochemistry was used to detect the expression of UBQLN2 in NSCLC cancer (24 cases) and adjacent normal tissues (24 cases), and to analyze the relationship between the expression of UBQLN2 and lymph node metastasis of NSCLC cancer. The expression of UBQLN2 in human normal bronchopulmonary epithelial cells and NSCLC cells was detected by real-time quantitative polymerase chain reaction (qRT-PCR)and Western blot; the effect of UBQLN2 on the proliferation of NSCLC cells was detected by lentivirus overexpression technology combined with MTS and EDU experiments in n vitro; the effect of UBQLN2 on the invasion and metastasis of NSCLC cells was detected by scratch experiments in n vitro and transwell experiments; a dual-fluorescence autophagy flow detection system was constructed by GFP-LC3-RFP-mLC3 plasmid packaging virus and Western blot was used to detect the change of autophagy after overexpression of UBQLN2; TCGA online data was uesd to analyze the expression level of UBQLN2 and lung cancer patients relevance of prognosis.n Results:The expression of UBQLN2 in normal lung tissues was significantly higher than that in NSCLC tissues (n P<0.01), and the expression in patients with negative lymph node metastasis was significantly higher than that in patients with positive lymph node metastasis (n P<0.01); the expression of UBQLN2 in NSCLC cells was significantly lower than that in normal lung epithelial cells, and the overexpression of UBQLN2 could inhibit the proliferation and invasion and metastasis of tumor cells. The expression of UBQLN2 was positively correlated with the prognosis of NSCLC.n Conclusions:The expression of UBQLN2 is significantly lower in lung cancer tissues and cells, and is negatively correlated with the lymph node metastasis of NSCLC; UBQLN2 can inhibit the proliferation and invasion of NSCLC cells; the expression of UBQLN2 is positively correlated with the prognosis of patients.