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构建以ＣＥＡ启动子控制ＨＳＶＴＫ基因表达的复制缺陷型腺病毒载体（ＡｄＣＥＡＴＫ）．纯化的重组腺病毒滴度达１×１０１２ｐｆｕ／ｍｌ．ＣＥＡ阴性的ＨｅＬａ细胞感染ＡｄＣＭＶＴＫ后对丙氧鸟苷（ＧＣＶ）很敏感，而感染了ＡｄＣＥＡＴＫ后不被ＧＣＶ杀伤．与此相反ＣＥＡ阳性的ＬｏＶｏ细胞中ＡｄＣＭＶＴＫ和ＡｄＣＥＡＴＫ都有很好的表达活性，说明ＣＥＡ启动子有良好的细胞专一性．ＡｄＣＥＡＴＫ／ＧＣＶ系统还有明显的旁杀伤效应．此载体将有助于实现对ＣＥＡ阳性肿瘤的专一性自杀基因治疗．
To construct a replication-defective adenovirus vector (AdCEATK) which controls the expression of HSVTK gene with CEA promoter. The purified recombinant adenovirus titer reached 1×1012 pfu/ml. CEA-negative HeLa cells infected with AdCMVTK were sensitive to GCV and were not killed by GCV after infection with AdCEATK. In contrast, AdCMVTK and AdCEATK were positively expressed in CEA-positive LoVo cells, indicating that the CEA promoter has good cell specificity. The AdCEATK/GCV system also has a significant side kill effect. This vector will help achieve a unique suicide gene therapy for CEA-positive tumors.