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目的 :研究腺病毒介导FasLigand基因转染对大鼠血管平滑肌细胞凋亡的影响。 方法 :常规分子生物学技术构建重组腺病毒Ad FasL、Ad βgal。免疫组化方法检测转染Ad FasL的大鼠血管平滑肌细胞FasLigand蛋白表达。用TUNEL法检测转染腺病毒的血管平滑肌细胞是否发生凋亡。结果 :10 0moiAd FasL转染大鼠血管平滑肌细胞 ,10 0 %细胞表达FasLigand蛋白 ,并发生凋亡。结论 :大鼠对Ad FasL转染诱导的凋亡易感。因此可以推断Ad FasL转染损伤后血管壁将减少血管平滑肌细胞积累。Ad FasL转染可能是有效的再狭窄治疗策略
Objective: To study the effect of adenovirus-mediated FasLigand gene transfection on the apoptosis of rat vascular smooth muscle cells. Methods: The recombinant adenovirus Ad FasL and Ad βgal were constructed by conventional molecular biology techniques. The expression of FasLigand protein in rat vascular smooth muscle cells transfected with Ad FasL was detected by immunohistochemistry. TUNEL method was used to detect apoptosis of transfectant adenovirus vascular smooth muscle cells. RESULTS: After 10moiAd FasL was transfected into rat vascular smooth muscle cells, 10% of the cells expressed FasLigand protein and apoptosis occurred. Conclusion: The rat is susceptible to apoptosis induced by Ad FasL transfection. Therefore, it can be inferred that transfection of Ad FasL into the injured vessel wall will reduce the accumulation of vascular smooth muscle cells. Ad FasL transfection may be an effective restenosis treatment strategy