论文部分内容阅读
与抗原加工有关的转运子(TAP)基因产物位于内质网膜,将具有抗原性的多肽从胞液转移进内质网,与人白细胞抗原(HLA)I类分子及β2微球蛋白进行组装.TAP基因的多态性导致特定的多肽进入内质网膜.从而通过主要组织相容性复合物(MHC)分 子来影响抗原的提呈和针对这些抗原的免疫应答.作者以美国881名15月龄时免疫过麻疹-腮腺炎-风疹(MMR-Ⅱ)疫苗的5～12岁健康儿童作为研究对象,测定了其中32名对麻疹疫苗无应答(IgG抗体阴性)者和28名高应答者的TAP等位基因.还用Fisher提取试验对无应答者和高应答者的TAP基因型、等位基因和纯合率以及两者在已知TAP基因多态性位点上个别氨基酸的频率进行了比较.
The antigen-processing-related transporter (TAP) gene product is located in the endoplasmic reticulum membrane and transports the antigenic polypeptide from the cytosol into the endoplasmic reticulum to assemble with human leukocyte antigen (HLA) class I molecules and β2 microglobulin Polymorphisms of the TAP gene lead to the entry of specific polypeptides into the endoplasmic reticulum, thereby affecting the presentation of antigens and the immune response to these antigens by means of major histocompatibility complex (MHC) molecules. Month-old healthy children aged 5 to 12 years old who had been immunized with measles-mumps-rubella (MMR-II) vaccine were tested in 32 of them who were unresponsive to measles vaccine (IgG antibody negative) and 28 high responders Of the TAP allele.The TAP genotypes, alleles and homozygosity of non-responders and high responders, as well as the frequency of individual amino acids at both known TAP polymorphic sites, were also tested using Fisher’s extraction test A