论文部分内容阅读
目的 检测口腔癌中的人乳头瘤病毒 ( HPV) 16型 DNA和 Ha- rar癌基因的点突变 ,探讨HPV16型和 Ha- ras癌基因的点突变与口腔癌之间的关系。方法 应用多聚酶链反应 ( PCR)技术扩增HPV16DNA和 Ha- ras癌基因相关片段 ,分别采用 2 %琼脂糖凝胶电泳和限制性片段长度多态性分析( RFL P) ,检测口腔癌中的 HPV16DNA和 Ha- ras癌基因第 12位密码子的点突变。结果 在 17例口腔癌组织中 ,6例 ( 35 .5 % )组织 HPV16DNA阳性 ,5例 ( 2 9.4% )组织 Ha— ras癌基因发生点突变 ,其中 2例组织 HPV16DNA和 Ha- ras癌基因点突变的检测均为阳性。结论 口腔癌的发生可能与 HPV16和Ha— ras癌基因的点突变有一定的关系。
Objective To detect point mutations in human papillomavirus (HPV) 16 and Ha-rar oncogenes in oral cancer and explore the relationship between point mutations in HPV16 and Ha-ras oncogenes and oral cancer. Methods HPV16 DNA and Ha-ras oncogene-related fragments were amplified by polymerase chain reaction (PCR), and 2% agarose gel electrophoresis and restriction fragment length polymorphism (RFLP) were used to detect HPV16 DNA And point mutation at codon 12 of the Ha-ras oncogene. Results In 17 cases of oral carcinoma, 6 cases (35.5%) had HPV16 DNA positive and 5 cases (2 9.4%) had mutations in Ha-ras oncogene, including 2 cases of HPV16 DNA and Ha-ras oncogene Mutations were positive. Conclusion The occurrence of oral cancer may be related to the point mutation of HPV16 and Ha-ras oncogene.