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目的 :观察不同时期肝表面移植脾片的细胞免疫学情况以及移植前后脾片量的变化 ,对肝表面脾片移植进行综合评价。方法 :以Wistar大白鼠为研究对象 ,流式细胞仪检测移植脾片的T淋巴细胞总数、活化T细胞数、T辅助细胞数、T抑制细胞数。结果 :8周组CD3+CD8、CD4与对照组比 ,P <0 .0 5 ,4周组与任一组比 ,P <0 .0 1,脾片重量 6～ 8周均明显低于移植前 (P <0 .0 1)。结论 :肝表面移植脾片 6周及 8周各项T淋巴细胞数达到正常脾脏水平 ,脾片重量较移植前有所减轻。脾片成活理想。
OBJECTIVE: To observe the cellular immunology of splenic platelets in different stages of liver transplantation and the changes of splenomegaly before and after transplantation. Methods: The total number of T lymphocytes, the number of activated T cells, the number of T helper cells and the number of T-suppressor cells in splenic spleen were detected by flow cytometry in Wistar rats. Results: Compared with the control group, the CD3 + CD8 and CD4 in the 8-week group were significantly lower than those in the control group (P <0.05) Before (P <0. 01). Conclusion: The number of T lymphocytes in the spleen at the 6th and 8th weeks of liver transplantation reaches the normal spleen level, and the weight of the spleen is reduced compared with that before transplantation. Spleen survival ideal.