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本研究以分离自番茄、辣椒、草莓、葡萄的灰葡萄孢Botrytis cinerea为供试菌株,从生长温度、pH适应性、碳源、氮源营养利用等方面对不同寄主来源的灰葡萄孢菌株的生物学性状进行了比较研究。结果表明,5个不同寄主来源的灰葡萄孢菌株的菌丝生长温度范围相同,均为0-35℃;但它们的最适生长温度和分生孢子致死温度存在差异,来自和县番茄菌株HX12最适生长温度为20℃,分生孢子致死温度为47℃ 10min,其余最适生长温度均为25℃,分生孢子致死温度均为48℃ 10min;不同菌株在相同温度下的生长速率有显著差异。pH对不同寄主来源灰葡萄孢菌株菌丝生长的影响存在差异,来自长丰辣椒的菌株LJ菌丝在pH2-9的范围内均能生长,以在pH3-6.5时生长较快,pH6时最快;其余4个菌株在pH2-12的范围内均能生长,以在pH3-9时生长较快,pH6左右最快。不同碳源、氮源营养对灰葡萄孢菌株菌丝生长和分生孢子产生均有显著影响,不同寄主来源的菌株间在碳源、氮源营养利用差异均极显著。在相同碳源、氮源营养条件下,不同寄主来源的菌株的线性生长、菌丝干重和分生孢子产量均有显著差异。
In this study, Botrytis cinerea isolated from tomato, peppers, strawberries and grapes was used as test strain. The effects of growth temperature, pH adaptability, carbon source and nutrient utilization on different host strains of Botrytis cinerea Biological traits were compared. The results showed that the mycelial growth temperature range of Botrytis cinerea from five different host plants were the same from 0 to 35 ℃, but the difference between their optimal growth temperature and the lethal temperature of conidia was from the strain HX12 The optimal growth temperature was 20 ℃, the lethal temperature of conidia was 47 ℃ for 10min, the other optimum growth temperature was 25 ℃ and the lethal temperature of conidia was 48 ℃ for 10min. The growth rate of different strains at the same temperature was significant difference. The effects of pH on the mycelial growth of the different host strains of Botrytis cinerea were different. The strain LJ from Changfeng pepper could grow in the range of pH2-9 to grow faster at pH3-6.5 and fastest at pH6 ; The other 4 strains can grow within the range of pH2-12, so that they grow faster at pH3-9 and fastest at pH6. Different carbon sources and nitrogen sources had significant effects on the mycelial growth and conidia production of the strains of Botrytis cinerea, and the differences of nutrient utilization among carbon sources and nitrogen sources of different host strains were extremely significant. Under the same carbon source and nitrogen source, the linear growth, the dry weight of mycelia and the conidia production of different host strains were significantly different.