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为了解非污染区普通人群尿液中多环芳烃羟基代谢产物的水平,探讨其在非污染区人群中的内暴露情况,为污染区人群中多环芳烃的负荷研究提供基础资料。于2014年9月在农村非污染区随机选取63名村民为研究对象,收集其晨尿中段,采用高效液相色谱法检测尿液中多种单羟基多环芳烃的浓度。结果显示成人尿样中1-羟基芘、3-羟基苯并[a]芘、1-羟基菲+9-羟基菲、2-羟基菲+3-羟基菲、1-羟基萘、2-羟基萘、2-羟基芴、9-羟基芴的检出率分别16.28%,4.65%,23.26%,27.91%,55.81%,65.12%,97.67%,44.19%;平均浓度分别为2.6205,3.9894,0.5424,0.5644,150.339 4、7.508 3、2.232 4、35.728 3μg/g cr。儿童尿样中1-羟基芘和3-羟基苯并[a]芘未检出;1-羟基菲+9-羟基菲、2-羟基菲+3-羟基菲、1-羟基萘、2-羟基萘、2-羟基芴、9-羟基芴的检出率分别为15%,25%,60%,35%,100%,45%,平均浓度分别为0.350 6、0.119 4、159.279 8、1.571 4、1.789 2、28.082 8μg/g cr。其中,成人尿样2-羟基萘的检出率和检出水平高于儿童(P<0.05)。提示非污染区普通人群尿样OH-PAHs均有一定的检出率;成人和儿童OH-PAHs水平存在差异,为进一步开展PAHs代谢产物在污染区人群的暴露提供了基础数据。
In order to understand the level of hydroxyl metabolites of polycyclic aromatic hydrocarbons (PAHs) in the urine of non-polluted areas and investigate their internal exposure in non-polluted areas, this study provides the basic information for the study of PAHs in polluted areas. In September 2014, 63 villagers were randomly selected from non-contaminated areas in rural areas to collect their morning urine. The concentrations of various monohydroxy PAHs in urine were measured by high performance liquid chromatography (HPLC). The results showed that 1-hydroxy-pyrene, 3-hydroxybenzo [a] pyrene, 1-hydroxy-phenanthrene + 9-hydroxy-phenanthrene, 2-hydroxy-phenanthrene + 3- , 2-hydroxyfluorene and 9-hydroxyfluorene were 16.28%, 4.65%, 23.26%, 27.91%, 55.81%, 65.12%, 97.67% and 44.19% respectively. The average concentrations were 2.6205, 3.9894, 0.5424 and 0.5644 , 150.339 4,7.508 3,2.232 4,35.728 3 μg / g cr. 1-hydroxy-phenanthrene + 9-hydroxy-phenanthrene, 2-hydroxy-phenanthrene + 3-hydroxy-phenanthrene, 1-hydroxynaphthalene, 2-hydroxy The detection rates of naphthalene, 2-hydroxyfluorene and 9-hydroxyfluorene were 15%, 25%, 60%, 35%, 100% and 45%, respectively. The average concentrations were 0.350 6,0.119 4,159.279 8,1.571 4 , 1.789 2,28.082 8 μg / g cr. Among them, the detection rate and detection level of 2-hydroxynaphthalene in adult urine samples were higher than those in children (P <0.05). It suggested that OH-PAHs in urine of common people in non-polluted areas had a certain detection rate. There were differences in OH-PAHs levels between adults and children, which provided the basic data for further studying the population exposure of metabolites of PAHs in polluted areas.