T-细胞核因子(NFAT)过度激活可引起免疫病理反应,如自身免疫、排斥反应和炎症。作者发现枫香树Liquidambar formosana果实的甲醇提取物对NFAT有强抑制活性,因而以生物活性为导向,从该植物果实中分离出4个齐墩果烷型三萜类化合物,研究了它们对转录因子NFAT的抑制活性。干燥、粉碎的该植物果实用甲醇提取,浓缩后混悬于水中,依次用己烷、乙酸乙酯和正丁醇分配提取。乙酸乙酯部位用硅胶柱层析, Excessive activation of T-cell nuclear factor (NFAT) can cause immunopathological reactions such as autoimmunity, rejection and inflammation. The authors found that the methanol extract of Liquidambar formosana fruit has strong inhibitory activity against NFAT. Therefore, based on biological activity, four oleanane triterpenoids were isolated from the fruit of the plant and their transcription was studied. Factor NFAT inhibitory activity. The dried, pulverized fruit of the plant was extracted with methanol, concentrated, suspended in water and partitioned and extracted with hexane, ethyl acetate and n-butanol. The ethyl acetate fraction was chromatographed on silica gel,