正常妇女尿中雌激素的含量极微,并被其它甾体代谢物所干扰,测定较为困难。过去都用 Brown 的化学法来测定,但过程繁而灵敏度也不高。自甾体化合物在气相色谱上应用分析后,Wotiz 即用来测定孕尿中的雌激素。在应用气相层析前,尿的处理是关键,一些人曾采取不同的处理办法。一般说来,孕尿中雌激素含量高(每天一毫克以上),较易测定,非孕尿的雌激素水平很低(每天只有几微克到几十微克),干扰物质就相对地很大,因此要求建立一个既能简便地去除杂质,又能正确定量的方法。近来利用普通氢焰检测的色谱仪测定雌激素的同类工作,大多是测妊娠后期雌三醇的变 Normal women urinary estrogen content is minimal, and other steroid metabolites interfere with the determination of more difficult. In the past using Brown’s chemistry to determine, but the complexity of the process is not high sensitivity. Wotiz is used to determine estrogen in the urine of the pregnant woman after the application of the steroid compound on the gas chromatograph. Before applying gas chromatography, urine treatment is the key, some people have taken a different approach. In general, high levels of estrogen in pregnant urine (more than one milligram per day) are easier to measure and the level of estrogen in non-pregnant urine is very low (only a few micrograms to tens of micrograms per day), interfering substances are relatively large, Therefore, it is required to establish a method that can remove impurities easily and correctly. Recently, the use of common hydrogen flame detection chromatographic apparatus to measure the similar work of estrogen, mostly pregnancy estriol measurement of change