论文部分内容阅读
利用费氏弧菌(Vibrio fisheri)发光原理研究了3种环境毒物对费氏弧菌的单一毒性与联合毒性的影响。结果显示,Cd~(2+)、除草剂草甘膦、除虫剂啶虫脒单独存在时,均对发光菌产生毒性作用,其光抑制率随测试时间的延长和浓度的增大而增强。但三者对费氏弧菌的剂量-效应关系曲线的影响有所不同,Cd~(2+)及啶虫脒的剂量-效应关系曲线趋于S型,而草甘膦的剂量-效应关系曲线为直线型。Cd~(2+)、草甘膦和啶虫脒对费氏弧菌的联合毒性与作用时间和体积比密切相关。当体积比为1:1时,Cd~(2+)与草甘膦的联合作用在5 min时表现为相加作用,15～45 min时表现为拮抗作用;Cd~(2+)与啶虫脒的联合作用在5min时表现为拮抗作用,15～30 min时表现为相加作用,45 min时表现为协同作用;草甘膦与啶虫脒的联合作用在整个测试时间段均表现为弱相加作用。从整个测试时间不同体积比来看,Cd~(2+)与草甘膦按0.1:0.1和0 2:0.5混合表现为拮抗作用,按1:1混合表现为相加作用;Cd~(2+)与啶虫脒按各比例混合均表现为相加作用;草甘膦与啶虫脒按各比例混合均表现为弱相加作用。
Using Vibrio fisheri luminescence principle, the effects of three kinds of environmental toxicants on the single toxicity and the combined toxicity of Vibrio fischeri were studied. The results showed that both Cd 2+ and glyphosate herbicide glyphosate and acetamiprid had the toxic effect on the luminescent bacteria, and their photo-inhibition rate increased with the increase of test time and concentration . However, the effects of the three on the dose-response curve of Vibrio fischeri were different. The dose-response curves of Cd 2+ and acetamiprid tended to S-shape, while the dose-response relationship of glyphosate The curve is straight. The combined toxicity of Cd 2+, glyphosate and acetamiprid to Vibrio fischeri was closely related to the time and volume ratio. When the volume ratio was 1: 1, the combined effect of Cd 2+ and glyphosate was additive at 5 min and antagonistic at 15-45 min. Cd 2+ and pyridine The combination effect of acetamiprid showed antagonism at 5 min, additive effect at 15-30 min, and synergistic effect at 45 min. The combined effect of glyphosate and acetamiprid showed as Weak additive effect. From the different volume ratios of the whole test time, Cd 2+ and glyphosate showed antagonistic effects when mixed with 0.1: 0.1 and 0 2: 0.5, +) And acetamiprid mixed by each ratio showed an additive effect; glyphosate and acetamiprid mixed by the ratio showed a weak additive effect.