论文部分内容阅读
目的建立以改进的高效液相色谱法测定保健食品中大豆异黄酮含量的方法。方法固体样品经70%乙醇超生提取30 min,过滤膜后进行液相色谱测定,液体样品过滤后直接进行液相色谱测定。色谱柱:Agilent ZORB-AX SB-C18(250 mm×4.6 mm,5μm);流动相:甲醇和1%磷酸水溶液梯度洗脱;流速:1.0 ml/min;柱温:30℃;二极管阵列检测器,定量波长254 nm。结果大豆苷、大豆黄素、染料木苷、大豆素、大豆黄苷、染料木素6种组分的回收率在97.5%～105.0%之间,RSD在0.46%～3.33%之间,6种组分检出限分别为0.60、0.57、0.26、0.28、0.55、0.27μg/ml。结论该方法简便、快速、重现性好。
Objective To establish a method for the determination of soybean isoflavone content in health food by improved high performance liquid chromatography. Methods The solid samples were extracted with 70% ethanol for 30 min. After the membrane was filtered through liquid chromatography, the liquid samples were filtered and directly measured by liquid chromatography. Column: Agilent ZORB-AX SB-C18 (250 mm × 4.6 mm, 5 μm); mobile phase: gradient elution with methanol and 1% phosphoric acid; flow rate: 1.0 ml / min; , Quantitative wavelength 254 nm. Results The recoveries of daidzin, daidzein, genistin, daidzein, daidzein and genistein were between 97.5% and 105.0% with RSDs ranging from 0.46% to 3.33% and six The detection limits of the components were 0.60,0.57,0.26,0.28,0.55,0.27μg / ml. Conclusion The method is simple, rapid and reproducible.