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作者用HPLC等度洗脱法分离了欧洲越桔Vaccinium myrtillus L.中的15种花青素苷。色谱条件:Develosil ODS-HG 5柱(4.6mm×150mm);流动相为含0.5％三氟醋酸的甲醇-水(20∶80);流速2mL/min;柱温40℃;检测波长520nm;进样量100μL。该植物中15种花青素苷在50min内完全分离。该法重现性好,经对20份样品的测定,每种花青素苷峰面积和由溶剂峰洗脱位置规定的相对迁移的平均相对误差分别为0.19％～3.85％和0.64％～0.77％。主要花青素苷翠
The authors isolated fifteen anthocyanins from Vaccinium vaccinium myrtillus L. by HPLC isocratic elution. Chromatographic conditions: Develosil ODS-HG 5 column (4.6 mm×150 mm); mobile phase methanol-water (20:80) containing 0.5% trifluoroacetic acid; flow rate 2 mL/min; column temperature 40° C.; detection wavelength 520 nm; Sample volume 100μL. Fifteen anthocyanins in this plant were completely separated within 50 min. The method has good reproducibility. After determination of 20 samples, the average relative error of each anthocyanin peak area and relative migration specified by the solvent peak elution position is 0.19% to 3.85% and 0.64% to 0.77%, respectively. . Major anthocyanin