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目的从海洋来源的马杜拉放线菌FIM95-F26发酵液中分离抗菌活性代谢产物。方法采用16S r RNA基因同源进化分析对放线菌FIM95-F26进行初步分类鉴定,同时对该菌Ⅱ型聚酮合酶基因进行同源进化分析。利用色谱分离技术获得活性代谢产物并对化合物进行结构鉴定和活性分析。结果与结论 16S r RNA基因序列分析表明菌株FIM95-F26属于马杜拉放线菌,而Ⅱ型聚酮合酶氨基酸序列的同源进化分析表明该菌具有产生芳香聚酮类化合物的潜力,通过色谱技术从其发酵液中分离到1个与IB-00208同质的多环氧杂蒽酮类化合物,活性分析表明该化合物具有较强的抗革兰阳性菌活性,其对短小芽孢杆菌、枯草芽孢杆菌、金黄色葡萄球菌和藤黄八叠球菌的MIC值分别为0.0312、0.125、0.125和0.25μg/m L,其中对短小芽孢杆菌和藤黄八叠球菌的抑制活性属首次报道。
OBJECTIVE To isolate antimicrobial active metabolites from the marine culture of Actinomyces moldula FIM95-F26. Methods The initial classification and identification of actinomycete FIM95-F26 were carried out by homologous evolution analysis of 16S rRNA gene. At the same time, homology evolution analysis of the type II polyketide synthase gene was carried out. The active metabolites were obtained by chromatographic separation and structural identification and activity analysis of the compounds. RESULTS AND CONCLUSIONS The 16S rRNA gene sequence analysis showed that strain FIM95-F26 belonged to Actinomadura, while homologous evolution analysis of the amino acid sequence of type II polyketide synthase showed that the strain had the potential of producing aromatic polyketides by Chromatographic techniques were isolated from its fermentation broth 1 and IB-00208 homogeneous polycyclic xanthone compounds, activity analysis showed that the compound has a strong resistance to Gram-positive bacteria, Bacillus pumilus, The MICs of Bacillus, Staphylococcus aureus and S. Amyloliquefaciens were 0.0312, 0.125, 0.125 and 0.25 μg / mL, respectively. The inhibitory activity against Bacillus pumilus and N. luteus was reported for the first time.