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目的:探讨由唐传(厦门)生物科技有限公司从水稻,玉米秸秆中提取的L-阿拉伯糖在降低空腹血糖,提高糖耐量及控制体重方面的重要生物学功能,从而为其进一步的功能食品开发提供科学依据。方法:以20g～22g昆明小鼠为实验动物,在给予高糖高脂饲料喂养的同时,采用高、中、低三种剂量口服L-阿拉伯糖水溶液一个月,以单独喂养高糖高脂饲料喂养组为对照,实验期间检测小鼠体重变化,食量。4周结束后进行小鼠空腹血糖及蔗糖糖耐量试验。结果:显示高剂量(250mg/只)和中剂量组(150mg/只)对小鼠空腹血糖有明显降低作用(P<0.05),而低剂量组(30mg/只)作用不明显,糖耐量试验证明三个剂量组的L-阿拉伯糖都具有明显增强小鼠糖耐量作用,与对照组有极显著差异(P<0.01),实验过程中未发现实验组与对照组小鼠食量有明显差异。解剖结果发现非用药组肝组织外观颜色淡黄色,而喂养L-阿拉伯糖组肝脏颜色为正常的深红色,肝指数变大,病理切片发现,实验对照组肝组织结构发生改变,肝细胞脂肪积累,PAS染色发现,肝脏切片染成深红色,而喂养L-阿拉伯糖组PAS染色成浅红色。结论:L-阿拉伯糖能够明显降低喂养高糖高脂饲料小鼠的空腹血糖,增强糖耐量,并且能够有效减缓体重增长趋势。组织切片和PAS染色证明L-阿拉伯糖可以有效抑制外源性糖在肝脏细胞中的积累及相应造成的脂肪堆积,进而对糖在体内转化成脂肪从而造成脂肪在体内的堆积也有重要的抑制作用。
Objective: To investigate the important biological functions of L-arabinose extracted from rice and corn stalks by Tang Chuan (Xiamen) Biotechnology Co., Ltd. in reducing fasting blood glucose, improving glucose tolerance and controlling body weight, and thus further functional foods. Development provides scientific basis. Methods: 20 to 22 g Kunming mice were used as experimental animals. When high-fat and high-fat diets were fed, high-, medium-, and low-dose L-arabinose aqueous solutions were orally administered for one month to feed high-sugar and high-fat diets alone. The feeding group was used as a control and the body weight and food intake were measured during the experiment. After 4 weeks, fasting blood glucose and sucrose glucose tolerance tests were performed in mice. Results: High-dose (250 mg/dose) and middle-dose (150 mg/dose) groups showed a significant decrease in fasting blood glucose (P<0.05), while the low-dose group (30 mg/dt) had no significant effect. Glucose tolerance test It was proved that L-arabinose in all three dose groups significantly enhanced the glucose tolerance in mice, which was significantly different from that in the control group (P<0.01). No significant differences in food intake between the experimental group and the control group were found during the experiment. The anatomical results showed that the non-drug group had a pale yellow appearance, while the liver color of the L-arabinose group was normal dark red, and the hepatic index became larger. The pathological section found that the liver tissue structure was changed in the experimental control group and the hepatocyte fat accumulated. PAS staining revealed that liver sections were stained dark red, whereas PAS stained with L-arabinose was light red. Conclusion: L-arabinose can significantly reduce the fasting blood glucose in mice fed with high-sugar and high-fat diets, enhance glucose tolerance, and can effectively reduce the weight gain trend. Tissue sectioning and PAS staining demonstrated that L-arabinose can effectively inhibit the accumulation of exogenous sugars in liver cells and the resulting accumulation of fat, which in turn has an important inhibitory effect on the conversion of sugars into fat in the body and resulting in accumulation of fat in the body. .