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胡椒碱、荜茇明宁碱和墙草碱是荜茇中主要的酰胺类成分,具有多种药理活性,为了阐明这3种成分在5个不同种属肝微粒体中代谢差异,应用超高效液相色谱串联双压线性离子阱静电场轨道阱质谱(UHPLC-LTQ-Orbitrap MS)采集这3种化合物的全扫描图谱及二级三级质谱图,获取碎片离子信息,结合其质谱裂解规律和代谢产物精确质谱数据,比较3种生物碱分别在人、恒河猴、比格犬、大鼠以及小鼠肝微粒体中的代谢差异,并快速鉴定出3个胡椒碱代谢产物、2个荜茇明宁碱代谢产物及1个墙草碱代谢产物。结果显示荜茇中酰胺类生物碱的主要代谢类型为苯环亚甲二氧基开环脱甲基和氧化反应,且在种属间存在代谢率差异,该研究为进一步阐明荜茇中胡椒碱类成分体内代谢途径提供了实验依据。
Piperine, 荜 茇 Minging base and pellitorin are the main amide components in 荜 茇, with a variety of pharmacological activities, in order to clarify the three components in five different species of liver microsomal metabolic differences, the application of ultra-high efficiency Liquid chromatography-tandem dual-pressure ion trap electrostatic field orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap MS) were used to collect the full-scan and second-order MS spectra of the three compounds and obtain the fragment ion information. Metabolite accurate mass spectrometry data were used to compare the metabolic differences between the three alkaloids in human liver, rhesus monkey, beagle dog, rat and mouse liver microsomes, and the rapid identification of three piperine metabolites, two 荜茇 Mingling alkali metabolites and 1 wall grass alkali metabolites. The results showed that the major metabolites of amide alkaloids in the 荜 茇 were benzene ring methylenedioxy ring-opening demethylation and oxidation, and there was a difference in metabolic rate among the species. In order to further elucidate the effects of piperine In vivo metabolic pathways provide the experimental basis.