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在获得耐抽薹大白菜—结球甘蓝4号染色体单体异附加系AC4的基础上,以其为母本分别与不同基因型的2个耐抽薹大白菜自交系和2个易抽薹自交系杂交,利用根尖染色体计数法结合结球甘蓝4号染色体相对于大白菜的特异分子标记鉴定,对杂交后代进行筛选,在每个杂交组合后代中分别获得了2个2n=21和2个2n=20植株。抽薹现蕾时间鉴定结果表明每个杂交组合后代中2n=21的植株均较2n=20的植株抽薹现蕾时间推迟。对杂交后代及其父本植株中Br FLCs的表达进行了分析,结果表明Br FLCs基因表达量不是影响大白菜抽薹现蕾的唯一主要因素,基因组背景对抽薹现蕾时间效应大于Br FLCs基因,同时外源结球甘蓝4号染色体的添加对Br FLCs基因表达的效应受大白菜基因组背景的影响。
Based on the accession of AC4, an anther-tolerant cabbage-tolerant cabbage C4 line, two inbred lines of resistant bolting Chinese cabbage and two easy-bolting inbred lines with different genotypes The hybrids were identified by root tip chromosome counting combined with the specific molecular markers of cabbage chromosome 4 relative to Chinese cabbage. The hybrid progenies were screened and 2 2n = 21 and 2 2n = 20 plants. Bolting bud time identification results show that 2n = 21 plants per hybrid combination off 2n = 20 bolting bolting time delay. The expression of Br FLCs in hybrids and their male parent plants was analyzed. The results showed that the expression of Br FLCs was not the only factor that affected the bolting and budding of Chinese cabbage. The genomic background had greater time-effect on bolting and budding than that of Br FLCs, The effect of the addition of exogenous cabbage 4 on the expression of Br FLCs gene is influenced by the Chinese cabbage genome background.