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目的:采用HPLC指纹图谱研究不同提取工艺对活络效灵丹加减方(HLXL)中化学成分变化的影响。方法:以獐牙菜苦苷、芍药苷、隐丹参酮和异欧前胡素等11个特征指纹峰为指标,采用HPLC分析测定,条件为YMC-Pack ODS-A色谱柱,流动相甲醇-水梯度洗脱,检测波长210 nm,流速0.8 mL.min-1,柱温30℃。系统考察不同溶剂(水、乙醇、丙酮)、不同提取方式(超声、回流、冷浸)及单提混合液和混提液对各特征指纹峰相对含量的影响。结果:不同工艺提取物中特征峰相对峰面积存在较大差异。溶剂种类和提取方式对HLXL成分的提取有较大影响。结论:该方法重复性、精密度、稳定性良好,可用于HLXL不同提取工艺的指纹图谱研究。
OBJECTIVE: To study the effects of different extraction processes on the chemical composition changes of HLXL by HPLC fingerprinting. Methods: Eleven fingerprint peaks of swertia glycoside, paeoniflorin, cryptotanshinone and isoimperatorin were used as indexes. The chromatographic conditions were YMC-Pack ODS-A, mobile phase methanol-water Gradient elution, detection wavelength 210 nm, flow rate 0.8 mL.min-1, column temperature 30 ℃. The effects of different solvents (water, ethanol, acetone), different extraction methods (ultrasonic, reflux, cold soak) and single-stage mixed liquid and mixed liquid on the relative content of each characteristic fingerprint peak were systematically investigated. Results: The relative peak area of the characteristic peaks in different process extracts is quite different. The type of solvent and extraction methods have a greater impact on the extraction of HLXL components. Conclusion: The method has good repeatability, precision and stability, and can be used to study the fingerprints of different extraction processes of HLXL.