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目的 探讨癫疒间 患儿血清一氧化氮 (NO)、一氧化氮合酶 (NOS)的变化及意义。方法 利用ELISA方法 ,测定 5 8例癫疒间 患儿 (癫疒间 组 )和 2 3名健康儿童 (对照组 )血清中NO、NOS的含量 ,并分组比较不同条件下其含量的变化。结果 癫疒间 组血清NO、NOS的含量分别为 (5 .86± 1.2 1) μmol/ml和 (2 8.2 6± 8.4 9)U/ml,较对照组的 (3.78± 0 .74 ) μmol/ml及 (17.86± 4 .5 8)U/ml明显升高 (P <0 0 1) ;发作近期为 (7.31± 1.2 7)μmol/ml和 (31.2 5± 11.35 )U/ml,明显高于发作间期 (4 .2 7± 0 .6 6 ) μmol/ml和 (2 4 .15± 7.85 )U/ml(P <0 0 1) ;癫疒间 组EEG异常者为 (7.18± 1.35 ) μmol/ml和 (34.4 8± 8.5 6 )U/ml,明显高于EEG正常者 (4 .0 4± 0 .75 ) μmol/ml和 (2 2 .85± 7.4 5 )U/ml(P <0 0 1) ;但与发作类型、病程及是否接受治疗无关 (P >0 0 5 )。结论 癫疒间 发作近期血中NO、NOS生成增加 ,NO作为内源性调质参与癫疒间 发作病理生理过程
Objective To investigate the changes and significance of serum nitric oxide (NO) and nitric oxide synthase (NOS) in children with epilepsy. Methods Serum levels of NO and NOS in 58 epileptic children (epilepsy group) and 23 healthy children (control group) were measured by ELISA, and their contents were compared in different groups. Results The levels of NO and NOS in epilepsy group were (5.86 ± 1.2 1) μmol / ml and (2 8.26 ± 8.49) U / ml respectively, which were significantly higher than those in control group (3.78 ± 0.74) μmol / ml, and (17.86 ± 4.58) U / ml were significantly higher (P <0.01). The incidence of seizure was (7.31 ± 1.2 7) μmol / ml and (31.2 5 ± 11.35) U / ml, (4.27 ± 0.66) μmol / ml and (24.15 ± 7.85) U / ml (P <0.01) in interictal epilepsy group, and those in epileptic group were (7.18 ± 1.35) μmol / ml and (34.4 8 ± 8.56) U / ml were significantly higher than those with normal EEG (4.04 ± 0.75 μmol / ml and (2.85 ± 7.45) U / ml respectively 0 0 1). However, it was not related to the type of attack, duration of disease and whether or not to receive treatment (P> 0.05). Conclusion Epileptic seizures in the recent increase in blood NO, NOS production, NO as endogenous quenching and tempering involved in the pathogenesis of epileptic seizures