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为了比较青蒿素(Artemisinin)和蒿甲醚(Artemether)对人鼻咽癌CNE-1细胞放射增敏作用的差异,取指数生长期的人鼻咽癌CNE-1细胞,采用细胞克隆形成法分别检测青蒿素和蒿甲醚对CNE-1细胞生长的抑制效应,比较两种药物抑制效应的差异性,并确定实验的药物浓度;将人鼻咽癌细胞分为对照组、单纯药物组、单纯照射组及联合治疗组。单纯药物组、联合治疗组分别分为青蒿素组和蒿甲醚亚组,射线照射剂量为0、2、4、6和8Gy,采用多靶单击数学模型拟合细胞存活曲线,取放射增敏比(SER)为达到相同生物效应时,单纯照射的剂量与联合治疗组的剂量之比。实验结果表明,两种药物对细胞的抑制作用均随着药物浓度的提高而增强,存在剂量依赖关系。青蒿素对CNE-1细胞的抑制作用高于蒿甲醚,测得青蒿素放射增敏比(SER)为1.272,蒿甲醚SER为1.481,表明蒿甲醚较青蒿素对人鼻咽癌CNE-1细胞具有更强的放射增敏作用。
In order to compare the difference of radiosensitization effect of Artemisinin and Artemether on human nasopharyngeal carcinoma CNE-1 cells, CNE-1 cells of exponential growth phase were taken and cultured by cell clone method The inhibitory effects of artemisinin and artemether on the growth of CNE-1 cells were detected, and the differences in the inhibitory effects between the two drugs were compared. The drug concentrations were determined. The human nasopharyngeal carcinoma cells were divided into control group, , Simple irradiation group and combined treatment group. The simple drug group and the combination treatment group were divided into artemisinin group and artemether subgroup respectively. The dose of irradiation was 0, 2, 4, 6 and 8 Gy. The survival curves of cells were fitted by multi-target single-click mathematical model, The sensitization ratio (SER) is the ratio of the dose of radiation alone to that of the combination therapy group to achieve the same biological effect. The experimental results show that the inhibitory effects of the two drugs on the cells with the drug concentration increased, there is a dose-dependent relationship. Artemisinin had higher inhibitory effect on CNE-1 cells than artemether. The measured SER of artemisinin was 1.272 and SER of 1.481, indicating that artemether is more effective than artemether on human nasopharyngeal carcinoma CNE-1 cells have a stronger radiosensitization effect.