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我们应用Dot-ELISA和间接血凝试验(IHA)同时检测囊虫病患者血清。 材科和方法 抗原制备 猪囊尾蚴囊液先以3000rpm离心10min,再经10000rpm离心30min,上清液即为囊液粗抗原,蛋白含量为8.49mg/ml。
We applied Dot-ELISA and indirect hemagglutination test (IHA) to detect cysticercosis patients sera. Materials and Methods Antigen Preparation Cysticercus cellulosae liquid first centrifuged at 3000rpm 10min, and then centrifuged at 10000rpm 30min, the supernatant is cyst fluid crude antigen protein content of 8.49mg / ml.