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目的为深入揭示镉对雄性生殖毒性作用及其机理,本实验观察了饮水染镉对雄性小鼠生育机能的影响。方法 36只封闭群ICR小鼠(雄性,4周龄左右)随机分成4组,每组9只,持续饮用添加镉(氯化镉)浓度分别为0(对照组)、25、50和100 mg/L的饮水,后分为8周和18周两个阶段与健康成年雌鼠合笼交配(雄∶雌=1∶3),观察雌鼠的受胎率、产仔数和胎儿性别等情况。结果随染镉剂量的增加,饮水染镉8周和18周后的雄鼠受配雌鼠的受胎率均呈下降趋势,卡方检验证明雄鼠饮水染镉可以引起所配雌鼠的受胎率显著性降低(P<0.05);染镉8周和18周后的雄鼠所配怀孕雌鼠的平均产活仔数随染镉剂量的增加均呈下降趋势,与对照组比较,50 mg Cd2+/L以上剂量组均出现显著性变化(P<0.05),但染镉8周和18周间相互比较,差异无统计学意义(P>0.05);染镉8周和18周后的雄鼠所配雌鼠产仔的性别分布经卡方检验均没有显著性影响(P>0.05)。结论慢性饮水染镉对雄性小鼠的生育机能具有毒性作用。镉对性别的控制作用并非是通过直接影响雄性动物的生殖系统功能或精子状态实现的。
Objective To reveal the effect of cadmium on male reproductive toxicity and its mechanism. The effect of cadmium in drinking water on the reproductive function of male mice was observed in this experiment. Methods 36 closed ICR mice (male, 4 weeks old) were randomly divided into 4 groups with 9 rats in each group. The concentrations of cadmium chloride (cadmium chloride) in continuous drinking groups were 0 (control group), 25, 50 and 100 mg / L of drinking water, then divided into 8 weeks and 18 weeks of two stages and healthy adult females cage mating (male: female = 1: 3), observed females conception rate, litter size and fetal sex and so on. Results With the increase of cadmium dose, the fertility rate of male mice fed with cadmium in drinking water for 8 weeks and 18 weeks showed a decreasing trend. The chi-square test showed that cadmium in male rats could significantly increase the pregnancy rate (P <0.05). Compared with the control group, the average number of surviving littermates of pregnant rats assigned to males at 8 and 18 weeks after Cd exposure showed a decreasing trend. Compared with the control group, 50 mg Cd2 + (P <0.05). However, there was no significant difference between the two groups at 8 weeks and 18 weeks (P> 0.05). Male rats at 8 and 18 weeks after cadmium exposure Gender distribution of female mice born by chi-square test showed no significant gender distribution (P> 0.05). Conclusion Chronic drinking water cadmium has a toxic effect on the reproductive function of male mice. The role of cadmium in controlling sex is not achieved through the direct effects on reproductive system function or sperm status of male animals.