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目的:探讨左归丸水煎液对D-半乳糖诱导骨髓间充质干细胞(MSCs)衰老模型的影响.方法:分离、培养大鼠MSCs,D-半乳糖诱导MSCs建立衰老模型.MTT法检测衰老MSCs活性;流式细胞术比较细胞增殖周期的变化;细胞衰老β-半乳糖苷酶染色和吉姆萨染色比较细胞衰老情况.结果:模型组MSCs增殖能力显著低于正常组和左归丸中剂量组,模型组停滞在G1期细胞数目显著高于正常组和左归丸中剂量组,进入DNA快速复制期的细胞显著低于正常组和左归丸中剂量组,模型组细胞衰老β-半乳糖苷酶染色阳性率显著高于正常组和左归丸中剂量组.结论:左归丸水煎液在一定程度上可促进衰老MSCs增殖,减少衰老细胞,提示左归丸水煎液对体外培养诱导的衰老MSCs有一定的抗衰老作用.
OBJECTIVE: To investigate the effect of Zuogui pill decoction on the aging model induced by D-galactose in MSCs.Methods: The MSCs were isolated and cultured, and MSCs were induced by D-galactose to establish the model of aging.MTT assay Aging MSCs were measured by flow cytometry.Comparison of the changes of cell proliferation cycle by flow cytometry and the senescence of cells by aging β-galactosidase staining and Giemsa staining.Results The proliferation of MSCs in model group was significantly lower than that in normal group and Zuogui Wan The number of cells arrested in G1 phase in the dose group and the model group was significantly higher than that in the normal group and the Zuoguiwan medium dose group. The cells entering the rapid DNA replication stage were significantly lower than those in the normal group and Zuoguiwan medium dose group, The positive rate of galactosidase staining was significantly higher than that of normal group and Zuoguiwan medium dose group.Conclusion: Zuo Gui Pui decoction can promote the proliferation of aging MSCs to a certain extent and reduce the number of senescent cells, suggesting that Zuogui pill decoction In vitro cultured MSCs induced by senescence have certain anti-aging effects.