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青蒿琥酯(AST)的免疫调节效应已被认可,但其具体作用的机制尚不明确。本研究通过变应性接触性皮炎(ACD)小鼠模型,初步探讨AST对ACD的免疫调节作用及可能的分子机制。采用药效学分析以及HE染色、半定量RT-PCR、Western blotting等方法分别检测AST对ACD相关细胞因子、转录因子及信号分子表达的影响。结果显示,局部应用AST能够抑制ACD小鼠耳肿胀度、脾指数、炎症细胞浸润以及IFN-γ、T-bet的表达,同时减弱NF-κB p65的表达。结果表明,AST能够通过抑制炎症反应和ACD小鼠免疫功能,调节机体的Th1/Th2失衡发挥作用,NF-κB可能是其作用靶点之一。
The immunomodulatory effect of artesunate (AST) has been recognized, but the exact mechanism of action is not clear. In this study, allergic contact dermatitis (ACD) mouse model, preliminary study of AST immune regulation of ACD and possible molecular mechanisms. The effects of AST on the expression of ACD-related cytokines, transcription factors and signaling molecules were detected by pharmacodynamic analysis and HE staining, semi-quantitative RT-PCR and Western blotting. The results showed that local application of AST could inhibit ear swelling, spleen index, inflammatory cell infiltration and the expression of IFN-γ and T-bet in ACD mice and attenuate the expression of NF-κB p65. The results showed that AST can play a role in the regulation of the imbalance of Th1 / Th2 in mice by inhibiting the inflammatory reaction and immune function of ACD mice. NF-κB may be one of its targets.