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目的建立高效液相-串联质谱法(HPLC-MS/MS)测定人体血浆中利多卡因(lidocaine,LDC)及其代谢物单乙基甘氨二甲基苯酰胺(MEGX)和甘氨二甲基苯酰胺(GX)的浓度。方法采用API3000型HPLC-MS/MS液质联用系统,Ultimate C18分析柱(50×4.6 mm,5μm),甲氧苄啶作为内标,流动相为甲醇∶5 mmol/L醋酸铵=50∶50(甲酸调pH5.0),流速0.2 mL/min。样品在碱性条件下用乙酸乙酯提取浓集后进样,选择性离子监测模式检测。结果 LDC的线性范围为15.625~2000 ng/mL,MEGX和GX的线性范围均为1.5625~200 ng/mL。LDC、MEGX和GX的最低定量限分别为:15.625、1.5625和1.5625 ng/mL。结论该方法具有快速简便、灵敏准确等特点,可满足利多卡因及其代谢物临床药物浓度测定的需要。
Objective To establish a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) method for the determination of lidocaine (LDC) and its metabolites monoethyl glycyrrhizinate (MEGX) Benzamide (GX) concentration. Methods The API3000 HPLC-MS / MS LC / MS system was used. The column was C18 (50 × 4.6 mm, 5 μm) with trimethoprim. The mobile phase consisted of methanol: 5 mmol / L ammonium acetate 50 (formic acid pH5.0), flow rate 0.2 mL / min. The samples were extracted with ethyl acetate and concentrated under alkaline conditions, and then detected by selective ion monitoring mode. Results The linear range of LDC was 15.625 ~ 2000 ng / mL. The linear range of MEGX and GX was 1.5625 ~ 200 ng / mL. The minimum limit of quantification for LDC, MEGX and GX was 15.625, 1.5625 and 1.5625 ng / mL, respectively. Conclusion The method is rapid, simple, sensitive and accurate and can meet the need of determination of clinical drug concentrations of lidocaine and its metabolites.