本研究观察VEGFA和VEGFC及其受体和angiopoietin 1 angiopoietin 2及其受体在发育第 3- 12周人胚胎肝的表达。在征得意外流产孕妇同意并签字后 ,收集其意外流产受精龄为第 3- 12周的人胚胎 ,4 %多聚甲醛固定 ,石蜡包埋切片 ,HE和免疫组织化学染色 ,光镜观察。结果表明 :在发育第 4周 ,肝由少量肝索组成 ,肝内未见造血细胞和血细胞。肝内大、圆、有核的原始血细胞于发育第 5周开始出现 ,并强表达VEGFA、flt 4和Tie 2 ,其数量在发育第 7周达高峰。第 11- 12周 ,阳性细胞数量减少。这类细胞仅于发育第 6周时短暂表达Flk 1。第 7- 12周 ,肝细胞表达VEGFC和flt 1,阳性反应产物积聚在细胞质中。发育第 5周到第 12周胚胎肝血管内 ,有angiopoietin 1和angiopoietin 2阳性反应的细胞存在 ,细胞形态同上述VEGFA、flt 4和Tie 2阳性细胞。angiopoietin 1和angiopoietin 2阳性反应较弱 ,Tie 2的免疫反应强。发育各期 ,肝细胞也弱表达angiopoietin 1和angiopoietin 2和Tie 2。发育各期血管内皮细胞为上述各种因子和受体阴性反应。结论 :发育第 7周之前和第 7周之后 ,VEGF家族及其受体在肝内造血灶的细胞表达模式不同。胎肝造血与肝细胞的发育可能相关。 This study was to observe the expression of VEGFA, VEGFC and its receptors and angiopoietin 1 angiopoietin 2 and their receptors during the 3rd to 12th week of human embryonic liver development. After consenting and signing the accidental abortion, pregnant women of unexpected abortion were collected from the 3rd to 12th week of human embryo, 4% paraformaldehyde fixed, paraffin embedded sections, HE and immunohistochemical staining, light microscopy. The results showed that in the fourth week of development, the liver consisted of a small amount of hepatic cord, and no hematopoietic cells and blood cells were found in the liver. Hepatic large, round, nucleated primitive blood cells began to develop in the fifth week of development, and strong expression of VEGFA, flt 4 and Tie 2, the number reached the peak in the seventh week of development. Week 11-12 weeks, the number of positive cells decreased. These cells transiently express Flk 1 only at week 6 of development. From week 7 to week 12, hepatocytes expressed VEGFC and flt 1, and the positive reaction products accumulated in the cytoplasm. From the 5th to the 12th week of development, there were positive cells of angiopoietin 1 and angiopoietin 2 in the hepatic vessels of embryos. The morphology of the cells was the same as that of VEGFA, flt 4 and Tie 2 positive cells mentioned above. Positive response to angiopoietin 1 and angiopoietin 2 was weak, and Tie 2 had a strong immune response. At each stage of development, hepatocytes also weakly express angiopoietin 1 and angiopoietin 2 and Tie 2. Development of vascular endothelial cells for the various factors and receptor-negative response. CONCLUSIONS: Before and after the 7th week of development, the expression patterns of VEGF family and their receptors in the hepatic hematopoietic lesion are different. Fetal liver hematopoiesis may be related to the development of hepatocytes.