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目的探讨骨肉瘤中D类三种周期素——D1、D2及D3mRNA的表达。方法运用逆转录聚合酶链反应(RTPCR)及半定量RTPCR方法检测正常软组织和骨肉瘤中3种周期素DmRNA表达。结果正常软组织中检测不到周期素D1mRNA的表达,而骨肉瘤组织中均可检测到,但多数表达较弱;正常软组织及骨肉瘤组织中均存在周期素D2、周期素D3mRNA的表达,骨肉瘤中周期素D2、周期素D3的mRNA表达均明显高于软组织中的表达。结论周期素D2、D3可能在骨肉瘤失控的增生中起主要作用。
Objective To investigate the expression of three D-type cyclin D1, D2 and D3 mRNA in osteosarcoma. Methods Reverse transcription polymerase chain reaction (RTPCR) and semi-quantitative RT-PCR were used to detect the expression of three cyclin D mRNAs in normal soft tissue and osteosarcoma. Results The expression of cyclin D1 mRNA was not detected in normal soft tissue, but in osteosarcoma tissue, most of the expression was weak. The expressions of cyclin D2 and cyclin D3 mRNA were all found in normal soft tissue and osteosarcoma. The mRNA expressions of cyclin D2 and cyclin D3 were significantly higher than those in soft tissue. Conclusion Cyclin D2 and D3 may play a major role in the uncontrolled hyperplasia of osteosarcoma.