吸烟是慢性阻塞性肺病(COPD)发病的重要危险因素之一。探讨烟雾刺激时支气管肺泡灌洗液(BALF)中细胞及一些酶活性的变化,有助于阐明COPD发病及寻找有效防治药物。实验用Wistar种大白鼠,雄性,体重150～180g。随机分为正常对照组和烟雾刺激组(置大鼠于特制箱内,点燃香烟,每日接受烟雾刺激2小时共15天);处死动物后气道灌流收集BALF(2ml生理盐水×3次),并作细胞计数测定蛋白含量,MDA含量、β-葡萄糖醛酸酶 Smoking is one of the important risk factors for the development of chronic obstructive pulmonary disease (COPD). To investigate the changes of cells and some enzyme activities in bronchoalveolar lavage fluid (BALF) when smoke is stimulated, it is helpful to clarify the pathogenesis of COPD and find the effective prevention and treatment of drugs. Experimental Wistar rats, male, weighing 150 ~ 180g. The rats were randomly divided into normal control group and smoke stimulation group (rats were placed in a special box, lit with cigarettes, smoked for 2 hours daily for 15 days). BALF (2ml saline × 3 times) , And the cell count determination of protein content, MDA content, β-glucuronidase