用真菌β-微管蛋白基因的丰余寡聚核着酸引物B1和B3,扩增了一段871bp的水稻恶苗病菌Fusariummoniliforme的β微管蛋白基因片段,进行了克隆和DNA序列测定,并根据该序列设计了Fmoniliformeβ-微管蛋白基因的特异性测序引物。经过对恶苗病菌对多菌灵具有不同抗性水平菌株的β-微管蛋白基因核着酸序的比较研究,表明Fmoniliforme的β微管蛋白的165,198,200和257位置氨基酸末发生突变,在克隆的片段内也未发现能引起氨基酸改变的核着酸突变。说明该菌对多菌灵产生抗性的分子机理与目前已知的其他真菌有所不同,有待进～步研究。 A fragment of β-tubulin gene of 871bp Fusarium moniliforme from Fusarium moniliforme of Fusarium moniliforme was amplified by using the oligonucleotide primers B1 and B3 of the fungal β-tubulin gene, and cloned and sequenced. This sequence was designed for Fmiliforme β-tubulin gene specific sequencing primers. A comparative study of the nucleotide sequence of the β-tubulin gene of strains with different levels of resistance to carbendazim indicated that the amino acids at positions 165, 198, 200 and 257 of the β-tubulin of Fmoniliforme were mutated at the end of the cloned No intraspecific mutations that caused amino acid changes were found in the fragment. Indicating that the molecular mechanism of the bacteria on the carbendazim and other currently known fungi are different, to be further study.