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目的了解脂蛋白脂酶(1ipoprotein lipase,LPL)基因HindⅢ多态性在人群中的分布及其与血脂、体质指数和膳食因素的关系。方法通过聚合酶链反应和限制性片段长度多态性(PCR-RFLP)方法分析比较高脂血症患者156例和健康对照154人LPL-HindⅢ位点多态性,同时进行体格检查、膳食调查及血脂谱项目测定。结果高脂血症组与健康对照组的基因型H+H+、H+H-和H-H-及等位基因H+和H-的构成差异无统计学意义,3种基因型及H+和H-的性别上差异亦无统计学意义;基因型H+H+与非基因型H+H+的血脂谱及BMI水平差异无统计学意义(P>0.05);膳食调查结果显示,高脂血症人群中基因型H+H+与非基因型H+H+的不可溶性膳食纤维摄入量分别为(7.34±3.25)和(8.78±3.22)g,2组比较差异有统计学意义(P<0.05)。结论LPL基因HindⅢ位点非H+H+的不可溶性膳食纤维摄入量较高,此位点多态性不足以构成高脂血症的遗传危险因素。
Objective To investigate the distribution of Hind Ⅲ polymorphism of lipoprotein lipase (LPL) gene in human population and its relationship with serum lipids, body mass index and dietary factors. Methods The polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method was used to analyze the polymorphism of LPL-Hind Ⅲ locus in 156 hyperlipidemic patients and 154 healthy controls. The physical examination, dietary survey And lipid profile determination of the project. Results The H + H +, H + H- and HH- genotypes and the allele H + and H- of the hyperlipidemia group and the healthy control group showed no significant difference. The genotypes of H +, H + and H- There was no significant difference in gender. There was no significant difference in the lipid profile and BMI between H + H + genotypes and non-genotype H + H + genotypes (P> 0.05). The results of the dietary survey showed that the hyperlipidemia (7.34 ± 3.25) and (8.78 ± 3.22) g for H + H + and H + H +, respectively. The differences between the two groups were statistically significant (P <0.05). Conclusion LPL gene Hind Ⅲ site non-H + H + insoluble dietary fiber intake is high, the site polymorphism is not enough to constitute a genetic risk factor for hyperlipidemia.