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目的:观察L-四氢巴马汀(L-THP)对羟考酮依赖大鼠不同脑区单胺递质含量的影响,探讨L-THP对抗羟考酮依赖的机制。方法:以连续递增给药建立羟考酮依赖大鼠模型,L-THP伴随给药,采用HPLC-ECD法测定大鼠前皮层、海马、纹状体和伏隔核内多巴胺(DA)和5-羟色胺(5-HT)含量的变化。结果:与空白对照组相比,羟考酮依赖的大鼠纳络酮催促戒断后前皮层内DA、高香草酸(HVA)和5-HT含量明显降低,伏隔核内DA、3,4-二羟基苯醋酸(DOPAC)、HVA和5-HT含量明显降低,海马内DA、DOPAC、5-HT含量没有明显变化,而5-吲哚乙酸(5-HIAA)明显升高,纹状体内DA及其代谢产物DOPAC、HVA以及5-HT、5-HIAA含量明显降低;L-THP(10,20,30mg·kg-1,ig)伴随给药,能不同程度抑制上述变化。结论:L-THP可抑制羟考酮依赖引起的不同脑区内单胺类递质含量的变化,提示L-THP对抗羟考酮依赖可能与调节多巴胺和5-HT系统相关。
Objective: To observe the effect of L-THP on monoamine neurotransmitter in different brain regions of oxycodone-dependent rats and to explore the mechanism of L-THP on oxycodone-dependent mechanism. Methods: Oxycodone-dependent rat model was established by continuous increasing dose. L-THP was administered with high dose. The content of dopamine (DA), 5 and 5 in rat prefrontal cortex, hippocampus, striatum and nucleus accumbens were measured by HPLC- - Serotonin (5-HT) content changes. Results: Compared with the blank control group, oxycodone-dependent rat naloxone induced a significant decrease of DA, HVA and 5-HT contents in the anterior cortex after withdrawal, and DA, 3, The contents of DOPAC, HVA and 5-HT in hippocampus were not significantly changed, while the contents of 5-HIAA and 5-HIAA in hippocampus were significantly increased The content of DA and its metabolites DOPAC, HVA and 5-HT and 5-HIAA in the body decreased obviously. L-THP (10,20,30 mg · kg-1, ig) could inhibit the above-mentioned changes with different degrees. CONCLUSION: L-THP can inhibit the changes of monoamine neurotransmitters in different brain regions induced by oxycodone, suggesting that the dependence of oxytocin on L-THP may be related to the regulation of dopamine and 5-HT system.