在pH 4.0 Britton-Robinson缓冲溶液中,室温条件下,刚果红和血红蛋白反应生成缔合微粒,使体系的共振散射信号明显增强,并在529 nm处有一最大的共振散射峰。在选定的条件下,血红蛋白质量浓度在0.060～1.680μg/mL范围内与ΔI呈良好的线性关系,据此建立了一种测定血红蛋白含量的新方法。该方法的线性回归方程为ΔI=39.24ρ-1.776,相关系数r为0.9998,检出限为7.0×10-3μg/mL,将方法应用于尿样中血红蛋白含量的检测,回收率为94.2%～95.2%。 Under pH 4.0 Britton-Robinson buffer solution, congo red and hemoglobin reacted at room temperature to form associative microparticles, which markedly enhanced the resonance scattering signal of the system and had a maximum resonance scattering peak at 529 nm. Under selected conditions, the concentration of hemoglobin in the range of 0.060 ~ 1.680μg / mL showed a good linear relationship with ΔI, thus a new method for the determination of hemoglobin content was established. The linear regression equation of the method was ΔI = 39.24ρ-1.776, the correlation coefficient r was 0.9998, the detection limit was 7.0 × 10-3μg / mL. The method was applied to the determination of hemoglobin in urine sample with the recovery of 94.2% 95.2%.