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目的:研究抗氧化微量营养素(antioxidantmicronutrients,AM)对1型糖尿病大鼠Th2型细胞因子IL4、IL10基因表达的影响,以探索改善胰岛功能,防止糖尿病发生与发展的营养学因素。方法:应用链脲佐菌素(STZ)小剂量、多次注射法诱导大鼠1型糖尿病模型。AMSe、VE、V、Cr通过饮食方法补充。外周血、脾脏淋巴细胞IL4、IL10基因表达检测应用流式细胞术。血糖及自由基代谢通过生化分析法完成。结果:补充AM组大鼠外周血及脾组织IL4、IL10阳性淋巴细胞百分比明显高于模型对照组(P<0.05,P<0.01),其中Se+VE+V+Cr组升高更明显;糖尿病模型组IL4、IL10表达处于最低水平。补充AM各组较模型组明显增加了胰岛素分泌储备(P<0.05)。与模型组相比,AM补充各组血糖水平显著降低,GSHPx活力水平升高,MDA含量明显下降(P<0.05)。结论:AM最适剂量与组合不仅可以调节糖尿病大鼠胰岛素代谢、减轻氧化侵袭,而且通过上调IL4、IL10等Th2型细胞因子表达减轻胰岛损害。
Objective: To study the effect of antioxidantmicronutrients (AM) on the gene expression of Th2 cytokines IL4 and IL10 in type 1 diabetic rats and to explore the nutritional factors that can improve the function of islets and prevent the occurrence and development of diabetes mellitus. Methods: The model of type 1 diabetes was induced by multiple injections of streptozotocin (STZ) in small doses. AMSe, VE, V, Cr supplemented by diet. Peripheral blood, spleen lymphocytes IL4, IL10 gene expression was detected by flow cytometry. Blood glucose and free radical metabolism by biochemical analysis. Results: The percentage of IL-4 and IL-10 positive lymphocytes in peripheral blood and spleen of rats in AM group was significantly higher than that in model control group (P <0.05, P <0.01), especially in Se + VE + V + Model group IL4, IL10 expression at the lowest level. Compared with the model group, AM supplementation significantly increased insulin secretion reserve (P <0.05). Compared with the model group, the levels of GSHPx and MDA in the AM supplementation group were significantly decreased (P <0.05). Conclusion: The optimum dose and combination of AM can not only regulate insulin metabolism and reduce oxidative invasion in diabetic rats, but also alleviate the damage of islets by up-regulating the expression of Th2 cytokines such as IL4 and IL10.