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目的:观察参附注射液(SFI)对肠缺血再灌注(IR)大鼠肾组织内血红素加氧酶(HO-1)、诱生型一氧化氮合酶(iNOS)表达的影响,探讨其肾保护作用机制。方法:采用钳闭大鼠肠系膜上动脉(SMA)缺血再灌注模型。健康成年雄性大鼠随机分为缺血再灌注+生理盐水(NS)组(IR+NS)、SFI预处理组(IR+SFI)和假手术组(C)。应用免疫组织化学方法和图像分析系统检测肾组织中HO-1和iNOS的表达和分布,观察各组血清肌酐、尿素氮,同时光镜观察肾脏组织病理形态学改变。结果:与C组比较,IR+NS组HO-1和iNOS表达显著增强(均P<0.01),血清肌酐、尿素氮明显增加(P<0.01);与IR+NS组比较,IR+SFI组HO-1表达明显升高而iNOS表达明显降低(均P<0.05),血清肌酐、尿素氮明显降低。结论:SFI明显减轻肠IR所致的肾组织的损伤,能诱导肠IR后肾组织中HO-1的表达,同时抑制iNOS的表达。
Objective: To observe the effect of Shenfu injection (SFI) on the expressions of heme oxygenase (HO-1) and inducible nitric oxide synthase (iNOS) in rat kidney after intestinal ischemia reperfusion (IR) Explore its mechanism of renal protection. Methods: A rat model of ischemia-reperfusion of superior mesenteric artery (SMA) was established. Healthy adult male rats were randomly divided into three groups: IR + NS group, SF + SFI group and sham operation group. Immunohistochemistry and image analysis system were used to detect the expression and distribution of HO-1 and iNOS in renal tissue. Serum creatinine and urea nitrogen were observed in each group. Pathological changes of kidney tissue were observed with light microscope. Results: Compared with group C, the expressions of HO-1 and iNOS in IR + NS group were significantly increased (all P <0.01), serum creatinine and urea nitrogen were significantly increased (P <0.01) HO-1 expression was significantly increased and iNOS expression was significantly lower (all P <0.05), serum creatinine, urea nitrogen decreased significantly. Conclusion: SFI can significantly reduce the damage of renal tissue induced by intestinal IR, and induce the expression of HO-1 in renal tissue after IR and inhibit the expression of iNOS.