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目的：:观察局部应用螺旋藻多糖提取物（PSP）对大白兔金黄色葡萄球菌性角膜炎的治疗效果。方法：:实验研究。从钝顶螺旋藻干粉中提取PSP，并制备0.01% PSP滴眼液。在45只大白兔右眼角膜中央采用基质注射针基质注射5 μl金黄色葡萄球菌菌液[（ATCC25923，约100个菌落形成单位（CFU）]，构建兔金黄色葡萄球菌性角膜炎模型。角膜基质注射菌液8 h后，将兔随机分成基底对照组、0.9%氯化钠溶液组和PSP组3组，每组15只。0.9%氯化钠溶液组和PSP组分别给予0.9%氯化钠溶液或PSP点眼给药，每15 min点眼1次，持续点眼5次后，改为每30 min点眼1次，持续点眼14次，最后1次点眼后1 h，角膜荧光素钠染色，裂隙灯显微镜下观察兔实验眼角膜上皮缺损情况并给予临床评分，角膜取材后对CFU进行测定；每组随机取3只眼球进行组织病理学观察，采用实时定量PCR检测角膜中炎症因子的表达。组间数据比较采用独立样本n t检验。n 结果：:0.9%氯化钠溶液组角膜上皮缺损严重，PSP组角膜上皮缺损较少。与0.9%氯化钠溶液组相比，PSP组临床指标评分明显降低，差异有统计学意义（n t=5.293，n P<0.001）。与0.9%氯化钠溶液组相比，PSP组的CFU明显减少，差异有统计学意义（n t=4.383，n P<0.001）。组织病理学结果显示0.9%氯化钠溶液组有明显的炎症细胞浸润；PSP组与0.9%氯化钠溶液组相比，眼部结构相对良好，炎细胞浸润较少。实时定量PCR结果显示PSP组角膜组织中炎症因子白细胞介素-6、白细胞介素-1β、肿瘤坏死因子-α的表达明显低于0.9%氯化钠溶液组。n 结论：:0.01% PSP滴眼液能明显降低金黄色葡萄球菌性角膜炎病变的严重程度和角膜细菌载量，提示PSP对金黄色葡萄球菌性角膜炎可能具有潜在的治疗价值。“,”Objective::To observe the therapeutic effect of the local application of polysaccharide extract from spirulina platensis (PSP) on experimental Staphylococcus aureus keratitis in rabbits.Methods::In this experimental research, PSP was extracted from the powder of Spirulina platensis and 0.01% PSP eye drops were prepared. In 45 experimental rabbits, 5 μl of Staphylococcus aureus bacterial solution [ATCC25923, 100 corneal forming units (CFU)] was injected into the central cornea stroma with a matrix injection needle. Eight hours after corneal stroma injection, the rabbits were randomly divided into three groups: basal control group, 0.9% sodium chloride solution group and PSP group. The 0.9% sodium chloride solution group and the PSP group were given the 0.9% sodium chloride solution or PSP eye drops, respectively, once every 15 minutes, 5 consecutive times, then changed to once every 30 minutes, 14 consecutive times. One hour after the last eye drops, the corneal epithelial defects were evaluated under a slit lamp using fluorescein sodium staining and clinical scores were calculated. Then the corneas were acquired and CFU was determined. Three eyeballs in each group were randomly selected for histopathological observation, and real-time quantitative PCR was used to detect the expression of inflammatory factors in the cornea. The independent sample n t test was used for comparison between groups.n Results::The corneal epithelial defect was serious in the 0.9% sodium chloride solution group and less serious in the PSP group. Compared with the 0.9% sodium chloride solution group, the clinical score of the PSP group was significantly lower, and the difference was statistically significant (n t=5.293, n P<0.001). Compared with the 0.9% sodium chloride solution group, the quantity of Staphylococcus aureus bacteria and CFU in the PSP group decreased significantly (n t=4.383, n P<0.001). Histopathological results showed significant inflammatory cell infiltration in the 0.9% sodium chloride solution group. Compared with the 0.9% sodium chloride solution group, the PSP-treated group had relatively good ocular structure and less inflammatory cell infiltration. Real-time quantitative PCR results showed that the expression of interleukin-6, interleukin-1β, tumor necrosis factor-α was significantly lower in the PSP-treated group than that in the 0.9% sodium chloride solution group.n Conclusions::0.01% PSP eye drops can significantly reduce the severity and corneal bacterial load of Staphylococcus aureus keratitis, suggesting that PSP may have potential therapeutic value for Staphylococcus aureus keratitis.