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为筛选常温高效产酶复合菌系,分别以枯叶土壤、牛粪、堆肥和自然腐烂的小麦秸秆为微生物源,通过“外淘汰法”筛选了4组常温分解小麦秸秆并产胞外酶的复合菌系WSD-5、N、M和D。培养15d后,WSD-5分解小麦秸秆75.6%,而N、M和D的分解率分别为65.0%、69.4%和67.5%。通过各复合菌系对小麦秸秆纤维素、半纤维素和木质素成分的分解比较,可知WSD-5复合菌系具有明显的分解优势:相对于初始秸秆,WSD-5复合菌系使麦秆中的纤维素、半纤维素和木质素分别减少了94.2%、81.9%和21.3%。4组复合菌系虽然均检测到纤维素酶、木聚糖酶及滤纸酶的酶活性,但以WSD-5复合菌系的酶活性为最高。其中滤纸酶活性最高为1.30U/mL,纤维素内切酶活性达4.35U/mL,外切酶活性达到0.60U/mL,β葡萄糖甘酶活性达到0.43U/mL,木聚糖酶活性达到15.16U/mL。特异引物PCR结果显示,WSD-5复合菌系由真菌和细菌共同组成。
In order to screen the efficient strains of enzyme producing enzymes at room temperature and high temperature, respectively, four groups of thermostable wheat straw were screened through extracellular elimination method using leaves, soil, manure, compost and naturally decaying wheat straw as microbial sources. Enzyme complex strains WSD-5, N, M and D. After 15 days of cultivation, WSD-5 decomposed wheat straw by 75.6%, while the decomposition rates of N, M and D were 65.0%, 69.4% and 67.5%, respectively. WSD-5 complex strains showed obvious decomposition advantages compared with the decomposition of cellulose, hemicellulose and lignin components of wheat straw by each compound strain. Compared with the original straw, WSD- Of cellulose, hemicellulose and lignin decreased by 94.2%, 81.9% and 21.3% respectively. Although the activity of cellulase, xylanase and filter paper enzyme were detected in the four groups, the activity of WSD-5 was the highest. Among them, the highest activity of filter paper was 1.30U / mL, the activity of cellulase was 4.35U / mL, the activity of exo-enzyme was 0.60U / mL, the activity of β-glucoamylase was 0.43U / mL, the activity of xylanase reached 15.16U / mL. Specific primers PCR results show that, WSD-5 complex bacteria by fungi and bacteria together.