牛下丘脑酸性丙酮、甲醇提取物经SeDhadex G25、混合床树脂脱盐及高效液相色谱ODS柱分离,得到了较满意的钠泵抑制因子制备物。其生物活性测定证明,对纯化制备的家兔肾髓质外层Na~+,K~+-ATP酶活性、人红细胞~(86)Rb摄取率、WKY大鼠肠系膜小功脉血管平滑肌细胞Na~+外流及~3H哇巴因结合率等具有明显的抑制作用。经氨基酸组分及质谱分析,该物质属于小分子肽,分子量约为315Da。该抑制因子的意义在于作为生理状态及伴随体液容积扩张的病理状态下的钠运输调节因子,它可能与高血压时体液介导的细胞钠运输失调有关。 Bovine hypothalamus acidic acetone and methanol extracts were separated by SeDhadex G25, mixed-bed resin desalting and high-performance liquid chromatography ODS column, and the more satisfactory sodium pump inhibitor preparation was obtained. The bioassay showed that the activity of Na ~ +, K ~ + -ATPase, the uptake of human erythrocyte ~ (86) Rb, the content of Na ~ ~ + Outflow and ~ 3H ouabain binding rate has obvious inhibitory effect. The amino acid composition and mass spectrometry analysis, the material belongs to a small molecule peptide, molecular weight of about 315Da. The significance of this inhibitory factor lies in the sodium transport regulators as a physiological condition and in the pathological conditions associated with dilated body fluid volume, which may be related to humoral-mediated dysregulation of sodium transport in cells during hypertension.