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目的建立高效液相色谱法测定四环素片含量。方法色谱柱为SUPELCO Discovery C18(250mm×4.6mm,5μm),流动相为0.1%磷酸-甲醇(40:60),检测波长为268nm,柱温为30℃,流速1.0ml/min。结果四环素进样量的线性范围为0.1～8.0μg,r=0.9983(n=7),平均回收率为99.92%,RSD=0.89%(n=6)。结论所用方法准确、灵敏、专属性好,可用于四环素片的质量控制。
Objective To establish a method for the determination of tetracycline tablets by high performance liquid chromatography. Methods The column was SUPELCO Discovery C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was 0.1% phosphoric acid-methanol (40:60). The detection wavelength was 268 nm. The column temperature was 30 ℃ and the flow rate was 1.0 ml / min. Results The linear range of tetracycline injection was 0.1 ~ 8.0μg, r = 0.9983 (n = 7). The average recovery was 99.92% and RSD was 0.89% (n = 6). Conclusion The method is accurate, sensitive and specific, and can be used for the quality control of tetracycline tablets.