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将酚灭活的点状气单胞菌和柱状屈桡杆菌菌苗按不同比例制备成二联菌苗，注射接种草鱼后，通过检测受免草鱼血清中的凝集抗体效价和攻毒试验，初步证明了由这两种菌制备的酚灭活菌苗，可刺激草鱼产生良好的免疫反应，免疫保护力达到５６．０％～７３．０％。
The phenolic inactivated strains of A. punctatus and C. coagulans were prepared into two bacterins at different ratios. After inoculated with grass carp, the titer and agglutination test of agglutinating antibody in serum of grass carp were tested, It was initially proved that the phenol-inactivated bacterin prepared by these two kinds of bacteria could stimulate the grass carp to produce a good immune response with the immunity protection reaching 56.0% -73.0%.