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目的 探讨白细胞介素 2 (IL 2 )激活的骨髓 (ABM )抗肿瘤作用的免疫学机理。方法 取恶性血液病缓解期患者骨髓 ,分离单个核细胞 (MNC) ,与IL 2共同孵育后 ,测定CD3-CD86 +、CD2 8+、CD8+CD2 8+、CD3-CD16 /CD5 6 +的细胞比例及上清液中肿瘤坏死因子α(TNF α) ,干扰素γ(IFN γ)的含量。结果 (1)培养 72小时后 ,实验组CD3-CD86 +细胞、CD2 8+细胞、NK细胞比例及上清中TNF α和IFN γ的含量均比对照组增高 (P <0 0 5 )。 (2 )培养 1周后 ,实验组CD3-CD86 +比对照组显著增高 (P <0 0 1) ,CD2 8+细胞、NK细胞比例、CD8+CD2 8+均增高 (P <0 0 5 )。结论 (1)ABM中CD3-细胞表面共刺激分子CD86表达明显增加 ,与ABM中特异性细胞毒性T淋巴细胞(CTLs)的生成有关。 (2 )ABM中活化B细胞、NK细胞、CTLs、TNF α和IFN γ也直接或间接地参与了ABM的抗肿瘤作用
Objective To investigate the immunological mechanism of antitumor effects of interleukin 2 (IL 2) activated bone marrow (ABM). Methods The bone marrow of patients with hematological malignancies was isolated and mononuclear cells (MNC) were isolated. After incubation with IL 2, CD3-CD86 +, CD28 +, CD8 + CD28 + CD3 + CD16 / CD56 + cells were assayed. The proportion and supernatant of tumor necrosis factor alpha (TNF alpha), interferon gamma (IFN gamma) content. Results (1) After 72 hours of culture, the proportions of CD3-CD86 + cells, CD28 + cells, NK cells, and TNFα and IFNγ in the supernatant of the experimental group were higher than those in the control group (P < 0.05). (2) After 1 week of culture, CD3-CD86 + was significantly higher in the experimental group than in the control group (P <0 01), and the proportion of CD28 + cells, NK cells, and CD8 + CD28 + were all increased (P <0 05). . Conclusions (1) The expression of CD86 on the surface of CD3-cells in ABM was significantly increased, which was related to the generation of specific cytotoxic T lymphocytes (CTLs) in ABM. (2) Activation of B cells, NK cells, CTLs, TNF α, and IFN γ in the ABM also directly or indirectly involved in the anti-tumor effect of ABM.