用经人IgA或IgA并用LPS免疫的BALB/C鼠脾细胞与SP2/0细胞融合,获得高阳性率并且建立了四株分泌抗人IgA McAb的杂交瘤细胞(1A2,1B4,1D55,2C11)。它们分别经过九次有限稀释连续培养九个月仍能持续地分泌高滴度抗体,并且抗体分泌量随克隆化次数的增多而增加。目前用间接ELISA法测定可达1:1.28×10~9。这些McAb与正常人血清进行微量免疫电泳实验在γ_1～β_2区形成一条清晰的沉淀线。用兔抗小鼠Ig G亚类血清鉴定它们分别属于IgG_1及IgG_2b。 BALB/C murine spleen cells immunized with human IgA or IgA and LPS were fused with SP2/0 cells to obtain a high positive rate and to establish four hybridoma cells secreting anti-human IgA McAb (1A2, 1B4, 1D55, 2C11) . They were able to secrete high-titer antibodies continuously after nine consecutive dilutions for nine consecutive months, and the amount of antibody secreted increased with the number of clones. At present, the indirect ELISA method can be used to measure 1:1.28×10-9. The microimmunoelectrophoresis experiments of these McAbs and normal human serum form a clear line of precipitation in the γ_1-β2 region. The rabbit anti-mouse Ig G subclass serum was used to identify them as IgG_1 and IgG_2b, respectively.