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【目的】探讨肥胖状态下脂肪组织基因组DNA甲基化及甲基转移酶表达改变以及n-3多不饱和脂肪酸(n-3PUFAs)的影响作用。【方法】使用30只3～4周龄C57BL/6J雄性小鼠,随机分为3组,每组10只。小鼠分别给予两种高脂饲料(脂肪含量为34.9%,供能比为60%)-n-6PUFAs(脂肪来源于葵花籽油)饲料和n-3PUFAs(脂肪来源于鱼油)饲料喂养14周;以正常脂饲料(脂肪含量为4.3%,供能比为10%)(脂肪来源于葵花籽油)为对照。喂养结束后对小鼠脂肪组织基因组DNA总甲基化以及甲基转移酶(DNMTs)进行测定。【结果】与正常脂饲料喂养小鼠相比,两组高脂饲料诱导肥胖小鼠的脂肪组织DNA总甲基化程度均明显升高,但n-3PUFAs高脂饲料组升高的程度显著低于n-6PUFAs组。n-6PUFAs高脂饲料诱导肥胖小鼠的脂肪组织DNMT1、DNMT3a和DNMT3b的表达均显著高于正常脂饲料组小鼠,而n-3PUFAs高脂饲料喂养小鼠脂肪组织中这3种酶的表达无变化。【结论】肥胖状态下脂肪组织基因组DNA甲基化程度升高;鱼油n-3PUFAs具有抑制DNA甲基化的作用。
【Objective】 To investigate the effect of obesity on genomic DNA methylation and methyltransferase expression and n-3 polyunsaturated fatty acids (n-3 PUFAs) in adipose tissue. 【Methods】 Thirty male C57BL / 6J mice aged 3 to 4 weeks were randomly divided into 3 groups with 10 in each. Mice were given 14 weeks of feeding with two high-fat diets (34.9% for fat and 60% for energy) - n-6PUFAs (fat derived from sunflower oil) and n-3 PUFAs (fat derived from fish oil) ; Normal fat feed (fat content of 4.3%, energy ratio of 10%) (fat derived from sunflower oil) as a control. The total genomic DNA methylation and methyltransferase (DNMTs) of mouse adipose tissue were measured after feeding. 【Results】 Compared with normal fat-fed mice, the total DNA methylation of adipose tissue was significantly increased in obese mice fed with high-fat diets, but the level of n-3PUFAs high fat diets was significantly lower In the n-6PUFAs group. The expression of DNMT1, DNMT3a and DNMT3b in obese mice fed n-6PUFAs high fat diet was significantly higher than that in normal fat diet mice, while the expression of these three enzymes in adipose tissue of mice fed n-3PUFAs high fat diet No change. 【Conclusion】 The adipose tissue genomic DNA methylation level is increased in obese state. Fish oil n-3PUFAs can inhibit the DNA methylation.